Abstract：Objective To explore the innate immune signaling pathway that involved inhigh expression ofDENV2-induced miR-146a. Methods SiRNAs were used to silence upstream proteins of signaling pathway, molecule inhibitors were used to block downstream factors, and then real-time PCR was performed to detect the change in the expression of miR-146a.Results DENV2-induced miR-146a expression decreased by 60% in IPS-1-silencing cells, but not in TLR3-silencing cells. Furthermore, DENV2-induced miR-146a expression was reduced by using specific inhibitors of NF-κB, IKK, p38 and JNK, but not by ERK. Conclusions DENV2-induced miR-146a expression is dependent on RIG-I/MDA5-IPS-1, p38, JNK and IKK/NF-κB, but notdependent on TLR3in upstreamand ERK in downstream. miR-146a may become a new target for the prevention and treatment of dengue virus infection, and eventually provides a new perspective for clinical treatment and prevention of dengue hemorrhagic fever or dengue shock syndrome.
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