Abstract：Objective To study the impact of environmental exposure to Internet bars and betel quid chewing on DNA damage of oral buccal mucosal cells. Methods Systematic sampling method was used to select 5 Internet bars in Yuelu District of Changsha City. Random sampling method was employed to select healthy males without smoking and drinking habits and aged 18-40 years in the Internet bars and communities where the Internet bars were located, and then all the participants were divided into the control group, the group of web-surfing in Internet bar,the group ofbetel quid chewing, and the group of web-surfing and betel quid chewing in Internet bar.Exfoliated buccal cell samples were collected from all the participants, and then single cell gel electrophoresis (SCGE) and buccal micronucleus cytome (BMCyt) assay were applied to detect DNA damage.Results The percentages of tail DNA (%tail DNA) and the micronucleus frequency (‰MN) were significantly higher in the groups of web-surfing in Internet bar and betel quid chewing than in the control group (both P<0.05) as well as significantly higher in the group of web-surfingand betel quid chewingin Internet barthan in the groups of web-surfing in Internet bar and betel quid chewing (both P<0.05). There existed a dose-response relationship between the degree of DNA damage and the cumulative time of web-surfing in Internet bar, the amount of betel quid chewing. Conclusions Environmental exposure to Internet bars and betel quid chewing can respectively lead to DNA damage in oral buccal mucosal cells; moreover, simultaneous exposure to both the factors will further increase the degree of DNA damage.
刘圆圆, 杨丽艳, 王竹, 徐克前. 网吧环境和槟榔致口腔颊黏膜细胞DNA损伤研究[J]. 实用预防医学, 2018, 25(7): 769-772.
LIU Yuan-yuan, YANG Li-yan, WANG Zhu, XU Ke-qian. DNA damage of oral buccal mucosal cells induced by environmental exposure to Internet bars and betel quid chewing. , 2018, 25(7): 769-772.
[1] 刘波, 邓芙蓉, 郭新彪, 等. 四种类型公共场所室内细颗粒物水平影响因素的研究[J]. 中华预防医学杂志, 2009, 43(8):664-668. [2] Tan C, Lu S, Wang Y, et al. Long-term exposure to high air pollution induces cumulative DNA damages in traffic policemen[J]. Sci Total Environ, 2017, 593:330-336. [3] 萧福元, 袁晟, 桂卓嘉, 等. 湖南地区食用槟榔流行病学研究[J]. 实用预防医学, 2011, 18(7):1218-1222. [4] 粟晓玲, 马金辉, 萧福元, 等. 嚼槟榔与口腔癌关系的病例对照研究[J]. 实用预防医学, 2016, 23(9):1059-1062. [5] Angadi PV, Rao SS. Areca nut in pathogenesis of oral submucous fibrosis: revisited[J]. Oral Maxillofac Surg, 2011, 15(1):1-9. [6] Szeto YT, Benzie IF, Collins AR, et al. A buccal cell model comet assay: development and evaluation for human biomonitoring and nutritional studies[J]. Mutat Res, 2005, 578(1-2):371-381. [7] Thomas P. Buccal micronucleus cytome assay[J]. Nat Protoc, 2009, 4(6):825-837. [8] 沈隽, 郑频频, 周曦斓, 等. 上海市长宁区部分网吧环境烟草烟雾浓度调查[J]. 中国健康教育, 2013, 29(6):496-498. [9] 高山. 网吧室内环境检测及分析研究[D].淮南:安徽理工大学, 2016. [10] 徐坤, 吴瑞, 陈超, 等. 模拟网吧环境对小鼠学习记忆能力的影响[J]. 徐州医学院学报, 2005, 25(3):238-239. [11] Edrissi B, Taghizadeh K, Moeller BC, et al. N(6)-formyllysine as a biomarker of formaldehyde exposure: formation and loss of N(6)-formyllysine in nasal epithelium in long-term, low-dose inhalation studies in rats[J]. Chem Res Toxicol, 2017, 30(8):1572-1576. [12] Mehta M, Chen LC, Gordon T, et al. Particulate matter inhibits DNA repair and enhances mutagenesis[J]. Mutat Res, 2008, 657(2):116-121. [13] Javed F, Chotai M, Mehmood A, et al. Oral mucosal disorders associated with habitual gutka usage: a review[J]. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2010, 109(6):857-864. [14] Kausar A, Giri S, Roy P, et al. Changes in buccal micronucleus cytome parameters associated with smokeless tobacco and pesticide exposure among female tea garden workers of Assam, India[J]. Int J Hyg Environ Health, 2014, 217(2-3):169-175. [15] Dasgupta R, Saha I, Pal S, et al. Immunosuppression, hepatotoxicity and depression of antioxidant status by arecoline in albino mice[J]. Toxicology, 2006, 227(1-2):94-104. [16] Chaturvedi P, Vaishampayan SS, Nair S, et al. Oral squamous cell carcinoma arising in background of oral submucous fibrosis: a clinicopathologically distinct disease[J]. Head Neck, 2013, 35(10):1404-1409.
2018, Vol. 25 
