Abstract：Objective To explore the expression of serum microRNA-21 (miRNA-21) in neonates with hypoxic-ischemic encephalopathy (HIE) and its significance in the occurrence and development of HIE in neonatal patients, and to provide a scientific basis for the early diagnosis and prognosis of HIE in neonates. Methods Seventy-nine neonates born in the First Affiliated Hospital of Xinxiang Medical College from March 2015 to November 2016 were divided into the HIE group (n=51) and the normal control group (n=28). 51 neonatal patients in the HIE group were treated with ganglioside, 5 ml of venous blood were collected from each neonate in the two groups within 24-48 hours of birth and after 15 days of therapy. The expression levels of serum miRNA-21 and hypoxia inducible factor-1α (HIF-1a) were detected by real-time fluorescence quantitative PCR. Results Before the treatment, the serum levels of miRNA-21 and HIF-1α were significantly higher in the HIE group than in the control group ((5.25±2.17) vs. (1.21±0.10), (8.76±3.23) vs. (1.28±0.10), both P<0.001). After 15 days of therapy, the serum levels of miRNA-21 and HIF-1α were significantly declined as compared with those before the treatment ((2.13±1.06)vs.(5.25±2.17), (3.64±3.34)vs. (8.76±3.23), both P<0.05). Conclusions High expression of serum miRNA-21 and HIF-1α is correlated with HIE in neonates, which may be used as biological indicators for the early diagnosis and prognosis of neonatal HIE.
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2018, Vol. 25 
