Abstract:Objective To investigate the susceptibility to commonly-used antimicrobial agents in clinically isolated Enterobacter cloacae in recent years, to determine the prevalence of isolates producing extened spectrum β-lactamases (ESBLs) and cephalosporinase (AmpC), and to provide reference for reasonable use of antibiotics in clinical practice. Methods We collected Enterobacter cloacae strains isolated from clinical specimens in the First Hospital of Hunan University of Chinese Medicine from January to November in 2016. The routine antibiotic susceptibility tests were conducted by Vitek-2 Compact fully automatic bacterial identification and drug susceptibility analyzer. ESBLs and AmpC were determined respectively by phenotypic confirmatory test and three dimensional test. Results A total of 107 non-repeatedly isolated Enterobacter cloacae strains were collected mainly from respiratory medicine department (26/107, 24.3%), bone traumatology department (21/107, 19.6%) and central ICU (20/107, 18.7%). The main specimens were sputum (38/107, 35.5%) and wound secretion (29/107, 27.1%). Enterobacter cloacae strains were highly resistant to penicillin, third-generation cephalosporins and cephamycins. The drug resistance rates to piperacillin/tazobactam, cefepime and imipenem were 13.1%, 22.4% and 0.9% respectively. 30 (28.0%) strains produced ESBLs, 35 (32.7%) strains AmpC, and 14 (13.1%) stains ESBLs and AmpC parallelly. The rates of resistance to commonly-used antimicrobial agents were higher in the stains produced both ESBLs and AmpC enzyme than in the non-enzyme-producing stains. Conclusions Producing ESBLs and AmpC enzyme acts the important mechanism to multidurg resistance of Enterobacter cloacae; and hence, it is of great value to detect enzyme-producing isolates for antibiotic therapy.
宁兴旺, 朱惠斌, 匡敏, 谢小兵, 钱纯. 107株阴沟肠杆菌产ESBLs和AmpC酶检测及耐药性分析[J]. 实用预防医学, 2018, 25(5): 534-537.
NING Xing-wang, ZHU Hui-bin, KUANG Min, XIE Xiao-bing, QIAN Chun. Detection of ESBLs and AmpC β-lactamase in 107 strains of Enterobacter cloacae and analysis of their drug resistance. , 2018, 25(5): 534-537.