Abstract： Objective To analyze the factors influencing the accuracy of determination Results of 3-chloropropane esters in vegetable oil by gas chromatography mass spectrometry so as to put forward effective control measures for acquiring accurate determination results. Methods Gas chromatography-mass spectrometry was used to determine 3-chloropropane esters in vegetable oil samples with pretreatment, including suitable quantitative ion selection, ether purification and adsorbent and dehydrant baking. Results After selecting quantitative ion m/z 453, purifying ether and baking adsorbent and dehydrant under 500℃ for 3 hours, the correlation coefficient of working curve was 0.9995, and the determination values of reagent blank and negative vegetable oil samples were all less than the detection limits. The levels of two parallel determination of 3-chloropropane esters in proficiency testing samples from Food Analysis Performance Assessment Scheme (FAPAS) were both within the acceptable range, and the relative standard deviation (RSD) was 4.6%. Conclusions We find the key factors influencing determination of 3-chloropropane esters in this study. The chromatographic peak area of 3-chloropropane esters can not be integrated accurately when selecting m/z 289 as quantitative ion, but no interference exists using m/z 453. The background of 3-chloropropanol, which exists in ether, adsorbent and dehydrant, can affect the determination of 3-chloropropane esters in vegetable oil samples,so it must be purified.
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2018, Vol. 25 
