Value of mean corpuscular volume, mean corpuscular hemoglobin and hemoglobin A2 detectionin the screening of thalassemia among population of reproductive age
TANG De-guo, TIAN Jie, YANG Shao-hui, WANG Ju, AI Yan-min, ZHANG Miao
Maternal and Child Health Hospital of Yongzhou City, Yongzhou, Hunan 425000, China
Abstract：Objective To explore an appropriate strategy for thalassemia screening by evaluating the correlations between the mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), hemoglobin A2 (HbA2) and thalassemia among population of reproductive age, so as to improve the detection rate of thalassemia and decline its missed diagnosis and misdiagnosis rates. Methods We retrospectively analyzed 2,002 samples (including 234 cases of confirmed thalassemia and 1,768 cases of non-thalassemia) from the molecular epidemiological survey of hemoglobin-related diseases from April to May in 2016 among residents in Yongzhou. The sensitivity, specificity and accuracy of single index and combined indices of MCV, MCH and HbA2 in the screening and genetic diagnosis of thalassemia were statistically analyzed. Results The sensitivity of MCV, MCH and HbA2 in parallel testing (87.18%) was significantly higher than that of the three indexes in series testing (59.83%), with a statistically significant difference (P<0.05). The specificity of MCV, MCH and HbA2 in series testing (99.55%) was obviously higher than that of the three index in parallel testing (87.16%), with a statistically significant difference (P<0.05). The sensitivity of MCV and MCH in parallel testing (79.06%) was slightly lower than that of MCV, MCH and HbA2 in parallel testing (87.18%). The specificity (95.13%) and diagnostic coincidence rate (93.26%) of MCV and MCH were slightly lower than those of the three indexes in series testing (99.55%, 94.91%), without statistically significant differences (all P>0.05). Conclusions It is of great value to screening thalassemia through detection of MCV, MCH and HbA2. Parallel testingwith MCV and MCH is the best way for thalassemia screening, with the advantages of simplicity, money-saving and efficiency. It is conducive to screening of thalassemia in grass-roots hosptials.
唐德国, 田洁, 阳少辉, 王具, 艾延民, 张苗. MCV、MCH和HbA2检测在育龄人群地中海贫血筛查中的价值[J]. 实用预防医学, 2017, 24(12): 1458-1461.
TANG De-guo, TIAN Jie, YANG Shao-hui, WANG Ju, AI Yan-min, ZHANG Miao. Value of mean corpuscular volume, mean corpuscular hemoglobin and hemoglobin A2 detectionin the screening of thalassemia among population of reproductive age. , 2017, 24(12): 1458-1461.
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