VP1 genetic characteristics and subgenotype of coxsackievirus A6 associated with hand,foot and mouth disease in Hunan Province, 2015-2016
HUANG Wei, CHEN Yu, CAI Liang, LUO Kai-wei, ZHANG Fan, ZHOU Shuai-feng, ZHANG Hong
Key Laboratory of Microbial Molecular Biology of Hunan Province, Hunan Provincial Center for Disease Controland Prevention, Changsha, Hunan 410005, China
Abstract：Objective To investigate the pathogenic features of coxsackievirus A6 (CV-A6) associated with hand, foot and mouth disease (HFMD) in Hunan Province during 2015-2016 so as to provide a basis for scientific prevention and control of HFMD. Methods The positive specimens of other enteroviruses sent by HFMD sentinel hospitals and prefecture-level CDCs in Hunan Province during 2015-2016 were collected to perform CV-A6 nucleic acid detection. CV-A6 real-time PCR positive specimens were then subjected to RT-PCR for full length sequence amplification and sequencing of VP1 region. The phylogenetic analysis was performed to analyze the full-length gene sequences encoding VP1 of CVA6 isolates and sequences downloaded from GenBank by using Seqman and MEGA 5.2 software packages. Results A total of 281 non-EV-A71 and non-CV-A16 enterovirus positive specimens were detected by CV-A6 real-time PCR assay. 142 specimens were positive for CV-A6, with the positive rate being 50.53%. 69 specimens from different regions and different years were selected for determining the full length sequence of VP1 region and 60 were successfully determined. It was found that all sequences detected belonged to CV-A6 subtype D cluster 1. Conclusions There are non-EV-A71 and non-CV-A16 enterovirus infections caused by CV-A6 in hand, foot and mouth disease in Hunan Province, and the isolates belong to the D1 subgenotype, which is consistent with the prevalent subtypes in other regions in China.
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2017, Vol. 24 
