三氯乙烯对肝细胞凋亡基因和肝代谢酶基因表达水平的影响

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摘要目的研究三氯乙烯（TCE）对人肝细胞凋亡基因（Caspase-3、Caspase-8、BAX、Bcl-2）和肝代谢酶基因(CYP1A2、CYP2E1、CYP3A4) mRNA表达水平的影响。方法用含10%胎牛血清的RPMI-1640 培养肝细胞,DMSO作为溶剂对照,采用不同浓度TCE（0.2、0.4、0.8、1.6、3.2 mmol/L）染毒L02肝细胞24 h,另外用TCE 1.0 mmol/L染毒细胞不同时间（2 h, 4h, 8h, 16h, 32h）,采用Real-time 荧光定量PCR技术检测肝细胞中凋亡基因和CYP基因mRNA表达水平。结果不同剂量TCE染毒后,凋亡基因Caspase-3、Caspase-8、BAX表达水平比对照组升高21%~145%,差异有统计学意义（P<0.05 或P<0.01）, Bcl-2表达水平下降20%~35%。TCE染毒引起CYP1A2、CYP3A4、CYP2E1的 mRNA表达明显上升,比对照组升高30%~550%,差异有统计学意义（P<0.05 或P<0.01）。用TCE 1.0 mmol/L染毒2 h~32 h,同样发现凋亡基因和肝代谢酶基因表达增加,与对照组比较差异有统计学意义（P<0.05,或P<0.01）。结论TCE可引起肝细胞凋亡基因和肝代谢酶基因表达变化,凋亡基因和肝代谢酶基因可能在TCE对肝细胞毒性效应中发挥重要作用。

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关键词 ：三氯乙烯, L02肝细胞, 凋亡基因, 肝代谢酶基因, 细胞色素P450

Abstract：Objiective:To study mRNA expression of apoptosis genes and hepatic metabolic enzyme genes in hepatocytes after treatment with trichloroethylene (TCE). Methods: L02 hepatocytes (L02 cells) were cultured with various doses of TCE (0.2, 0.4, 0.8, 1.6, and 3.2 mmol/L) for 24h, or the cells were cultured with a single dose of TCE (1.0 mmol/L) for different periods of time (2h, 4h, 8h, 16h, 32h), the control was treated with DMSO, real-time fluorescent PCR assay was applied for detection of mRNA expression of apoptosis genes (Caspase-3、Caspase-8、BAX、Bcl-2) and hepatic metabolic enzyme genes (CYP1A2, CYP2E1,CYP3A) . Results: The relative levels of mRNA expression of apoptosis genes and hepatic metabolic enzyme genes remarkably increased after TCE treatment at various doses (0.2, 0.4, 0.8, 1.6, and 3.2 mmol/L) for 24h, the elevation of mRNA expression in TCE treatment groups was significant in comparison with control (P<0.05, or P <0.01). Additionally, when the cells were treated with a single dose of TCE (1.0 mmol/L) for different periods of time (2h, 4h, 8h, 16h, 32h), the mRNA expression also increased significantly compared with control (P <0.05, or P <0.01). Conclusions: Trichloroethylene could induce alternation of mRNA expression of apoptosis genes and hepatic metabolic enzyme genes, these genes might play an important role in cytotoxicity of trichloroethylene.

Key words： trichloroethylene L02 cells apoptosis genes hepatic metabolic enzyme genes cytochrome P450.

作者简介: 廖日炎（1974~）男,大学本科,副主任医师,主要研究方向是职业卫生与疾病控制。E-mail: 2587289286@qq.com

引用本文:

廖日炎,徐新云. 三氯乙烯对肝细胞凋亡基因和肝代谢酶基因表达水平的影响[J]. 实用预防医学, 2015, 22(7): 873-876. LIAO Riyan,XU Xinyun. Study on mRNA expression of apoptosis genes and metabolism genes in hepatocytes after trichloroethylene treatment. , 2015, 22(7): 873-876.

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https://www.syyfyx.com/CN/ 或 https://www.syyfyx.com/CN/Y2015/V22/I7/873

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