Resultsof serological detection for Brucellosis of key occupational groups in Pingshan county from 2011 to 2014 analysed by the method 《Standard diagnosis of Brucellosis》 WS269-2007 ．Results Health education and behavior intervention for Brucellosis key occupational groups has been done continuously for 3 years, Brucellosis positive rate decreased from 7.94% to lower than 4.00%, it’s statistical significance（χ²=8.300,P=0.040）before and after intervention, it’s also statistical significance between small towns, age , gender and occupation distribution (χ²=12.583,P=0.014）;χ²=10.996,P=0.027;χ²=6.168,P=0.013;χ²=8.065,P=0.045）Conclusions．The behavior intervention method was effective for the Brucellosis positive rate decrease, so health education and behavior intervention for Brucellosis key occupational groups must be strengthened .