Abstract：【Objective】 To investigate the correlation between the combination of smoking and the polymorphisms of extracellular superoxide dismutase and cytochrome P450(CY)1A1-MspⅠgenes and oral cancer. 【Methods】 The genetic polymorphisms of EC-SOD and CYP1A1 -MspⅠwere detected by polymerase chain reaction(PCR) technique in peripheral blood leukocytes of 670 oral cancer cases and 670 non-cancer controls, and the correlation between smoking, the two metabolic enzymes genetic polymorphisms and oral cancer were analyzed. 【Results】 The frequencies of EC-SOD and CYP1A1 -MspⅠwere 39.40% and 68.81% in oral cancer cases,and 21.34% and 43.88% in healthy controls respectively. Statistical tests showed significant difference in the frequencies between the two groups(P<0.05). The risk of oral cancer with EC-SOD(C/G) was significantly higher than that of controls(OR=2.40,95%CI=1.73~4.32).The individuals who carried with CYP1A1-MspⅠ(m2/m2) had a high risk of oral cancer(OR=2.82, 95%CI=1.84~4.41). Combined analysis of the polymorphisms showed that percentage of EC-SOD(C/G)/CYP1A1-MspⅠ(m2/m2) in oral cancer and control groups was 30.60% and 7.16% respectively(P<0.05). The people who carried with EC-SOD(C/G)/CYP1A1-MspⅠ(m2/m2) had a high risk of oral cancer(OR=6.87, 95%CI=2.18~9.45). The smoking rate of the case group was significantly higher than that in the control group(P<0.05), and statistic analysis suggested an interaction between smoking and EC-SOD(C/G)/CYP1A1-MspⅠ(m2/m2) genotypes polymorphisms which increased risk of oral cancer (OR=44.48, 95%CI=17.63~62.07), the interaction between SI(SI>400) and EC-SOD(C/G)/CYP1A1-MspⅠ(m2/m2) genotypes polymorphisms increased risk of oral cancer(OR=248.81, 95%CI=175.46~384.37).【Conclusion】 EC-SOD(C/G) and CYP1A1-MspⅠ(m2/m2) are the risk factors in oral cancer．Smoking is also related to the susceptibility to oral cancer. There may be a synergetic interaction among EC-SOD(C/G), CYP1A1-MspⅠ(m2/m2) and smoking on the elevated susceptibility of oral cancer.
郝春波, 廖天安, 刘成, 陈小滨, 巩蕾. EC-SOD和CYP 1A1-MspⅠ基因多态性与口腔癌的相关性研究[J]. 实用预防医学, 2015, 22(10): 1160-1163.
Hao Chunbo, Deng W ei, Liao Tianan, Liu Cheng, Chen Xiaobin, Gong Lei. Correlation between EC-SOD and CYP 1A1-MspⅠ genes polymorphisms and oral cancer. , 2015, 22(10): 1160-1163.
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2015, Vol. 22 
