HUANG Chengwei, LIANG Changwei, LIANG Xianan, BI Zhiyou, ZENG Yi, LAO Xueqiong, PAN Lihua, YAO Yanfeng
Objective To understand the contamination status of enteroviruses in key activity venues for children during the epidemic period of hand, foot and mouth disease (HFMD) in Nanning City, and to provide a reference basis for implementing precision prevention and control in communities. Methods Six types of key activity venues for children in Nanning City were selected to investigate the contamination status of enteroviruses related to HFMD in April-June and September-October, 2021. Smear samples from surfaces easily touched by children in these activity venues were collected. Real-time fluorescent quantitative PCR was used to detect HFMD-related enteroviruses, and the results were statistically analyzed. Results The positive rate of enterovirus nucleic acid was 0.74% in 15,490 environmental smeared samples. Specifically, 2,515 samples were collected from fever clinics (consulting rooms), 2,515 from pediatric clinics (wards), 2,675 from vaccination clinics, 2,675 from nurseries and kindergartens, 2,555 from children’s playgrounds and 2,555 from public toilets, with the positive rates being 0.87%, 1.03%, 1.31%, 0.64%, 0.31% and 0.23% respectively. The positive rate of enteroviruses was found to be the highest in vaccination clinics, but the lowest in public toilets. There were statistically significant differences in the positive rates of enteroviruses among different activity venues (χ2=31.122, P<0.001). The positive rate of other enteroviruses was the highest, the overall differences in the positive rates among the various pathogen types were statistically significant (χ2=113.799, P<0.001). The positive rate of enterovirus was the highest in April, but the lowest in September, showing statistically significant differences in the positive rates among different months (χ2=19.177, P=0.001). Conclusion There were different degrees of enterovirus contamination in the external environment of key activity venues for children in Nanning City during the HFMD epidemic period. Therefore, environmental disinfection in these activity venues should be strengthened during the epidemic season.
