Abstract：ObjectiveTo study the stereotyping,drug resistance and the molecular epidemiological characteristics of Salmonella in Changsha. Methods70 Salmonella strains that isolated from stool specimens of foodborne disease and foods in Changsha were identified by stereotyping. The antimicrobial susceptibilities were determined by broth microdilution method and 14 antimicrobial agents were chosen and examined. Homology analysis was performed with PFGE according to the protocols of PulseNet.ResultsThe dominant Salmonella serovars were Salmonella typhimurium and Salmonella enteritidis, accounting for 51.43% and 7.15% respectively. In the antimicrobial susceptibilities testing, the highest resistant rates were found in Ampicillin (68.57%), Sulfadiazine (61.43%) and chloramphenicol (57.14%). 62.86% salmonella strains were multi-drug resistant isolates. And a significant difference was observed for multidrug resistance between Salmonella typhimurium and other Salmonella serovars. All the isolates were divided into 57 PFGE type according to similarity 100%. The similarity coefficient of 36 isolates of Salmonella typhimurium that divided into four Clusters were between 58.1% and 100%. ConclusionOur findings indicated that Salmonella typhimurium and Salmonella enteritidis were the dominant Salmonella serovars. And PFGE result showed that there were epidemic strains in Changsha. Additionally, antibiotic resistance of Salmonella was in serious situation.
2016, Vol. 23 
