Abstract：ObjectiveTo explore the effect of hepatitis B virus(HBV) on the expression of collagen triple helix repeat containing 1（CTHRC1）and its regulatory mechanism. MethodsHepG2 cells were co-transfected with CTHRC1 gene promoter and HBV infectious clone pHBV1.3 and plasmids carrying individual genes of HBV, luciferase activity was measured, mRNA and protein expression of CTHRC1 were measured by RT-PCR and western blot. ResultsHBV X protein can activate CTHRC1 gene promoter activity as well as upregulate CTHRC1 mRNA and protein expression in HepG2 cells. ConclusionsHBV upregulate the expression of CHTRC1 via HBV X protein.
蔡春林, 陈伟红, 卓菲, 龙冬玲, 黎剑华. HBV调节胶原三股螺旋重复蛋白1表达的机制探讨[J]. 实用预防医学, 2016, 23(1): 45-47.
CAI Chun-lin, CHEN Wei-hong, ZHUO Fei, Long DONG-ling, Li Jian-hua. Exploration of the regulatory mechanism of HBV on the expression of CTHRC1. , 2016, 23(1): 45-47.
[1] Takeshita S, Fumoto T, Matsuoka K, et al. Osteoclast-secreted CTHRC1 in the coupling of bone resorption to formation[J]. J Clin Invest, 2013, 123(9):3914-24. [2] Ma MZ, Zhuang C, Yang XM, et al.CTHRC1 acts as a prognostic factor and promotes invasiveness of gastrointestinal stromal tumors by activating Wnt/PCP-Rho signaling[J]. Neoplasia. 2014, 16(3):265-78, 278.e1-13. [3] Wang FB, Zhu CL, Liu X, et al. HBV inhibits apoB production via the suppression of MTP expression[J]. Lipids Health Dis, 2011, 11;10:207. [4] Dai Y, Cros MP, Pontoizeau C, et al. Downregulation of transcription factor E4F1 in hepatocarcinoma cells: HBV-dependent effects on autophagy, proliferation and metabolism[J]. Carcinogenesis, 2014, 35(3):635-50. [5] Rawat S, Clippinger AJ, Bouchard MJ. Modulation of apoptotic signaling by the hepatitis B virus X protein[J]. Viruses, 2012, 8;4(11):2945-72.
2016, Vol. 23 
