Abstract：Objective To study the etiology spectrum of hand, foot and mouth disease (HFMD) and genetic characteristics of coxasckievirus A16 in Chenzhou City, Hunan Province, 2014, and provide scientific data for HFMD control and prevention. Methods Clinical specimens of HFMD cases were collected from the sentinel hospitals of Chenzhou City in 2014. Viral RNA of human enterovirus type A71 (HEV71), coxasckievirus A16 (CVA16), A6 (CVA6), A10 (CVA10) and other enterovirus (HEV) were detected by fluorescent PCR. The genotyping of HEV were performed by sequencing of the VP1 regions. Phylogenetic analysis was performed by MEGA6. Results A total of 854 samples were detected, and 55.62% were enterovirus-postive, with the most frequent serotypes of CVA16(28.00%), HEV71(25.05%), CVA6(13.89%), CVA10(12.42%) and CVB5(0.84%) 20.63% enterovirus had unknown genotypes.Significant differences were found in the constituent ratios of various pathogens among different months, age and patients, but not between genders (P>0.05). Gene sequencing of 6 CVA16 isolates illustrated that the genotyp were all subtype B1. Conclusions The occurrence of HFMD in Chenzhou City in 2014 has obvious seasonal features. The main dominant pathogens are prevalent alternately. Their interaction with human immune is reflected in their distribution in months and age. The CVA16 isolates in Chenzhou City are homologous to strains circulating in other areas of mainland China.
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2016, Vol. 23 
