Abstract：Objective To explore the distribution of methylenetetrahydrofolate reductase (MTHFR) C677T gene polymorphism among 353 physical examinees in Wuhan region. Methods PCR chip-hybridization was used to detect MTHFR C677T genotypes in 353 physical examinees from Physical Examination Center, the People’s Hospital of Wuhan University from April to August 2015. According to the characteristics of gender and age of physical examinees, the distribution features of MTHFR C677T genotype and allele frequencies were analyzed, and then compared with the data about the Han ethnic physical examinees in other cities in China. Results Among the 353 physical examinees, MTHFR 677CC, 677CT and 677TT genotype frequencies were 36.3%(128/353), 48.4%(171/353) and 15.3%(54/353)respectively, and MTHFR 677T allele frequency was 39.5%. No statistically significant difference was found in the genotype distribution of MTHFR C677T between males and females (P=0.802). Among different age groups (except the age group of ≥70 years), MTHFR 677CT genotype was predominant and its genotype frequencies in the group of < 30 years, the group of ≥ 30 years and the group of ≥ 50 years were 47.9%, 43.2% and 55.7% respectively. No statistically significant difference was found in the genotype distribution of MTHFR C677T among different age groups(P=0.120). As compared with the Han ethnic physical examinees in Beijing and Qingdao regions, there were statistically significant differences in the distribution of MTHFR C677T genotype and allele frequencies in Wuhan region(P<0.05). Conclusions The distribution of MTHFR C677T gene polymorphism among the 353 physical examinees in Wuhan region is not affected by gender and age, but shows regional specificity.
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2016, Vol. 23 
