Abstract：Objective To explore the expression of miR-7 in myocardial hypoxia reoxygenation model and its effect on the apoptosis of myocardial cells. Methods Myocardial hypoxia reoxygenation model was established, and the expression level of miR-7 in myocardial cells was detected by reverse transcription polymerase chain reaction (RT-PCR). Myocardial cells were transfected with miR-7 mimics and inhibitors, and then RT-PCR was used to detect the transfection effect. Flow cytometry was employed to detect the effects of miR-7 mimics and inhibitors on myocardial apoptosis induced by hypoxia reoxygenation. Lactate dehydrogenase (LDH) kit was used to detect the LDH level, and the protein expression levels of cleaved caspase-3 and p-Akt in cells were detected by Western blot. Results The expression level of miR-7 in hypoxia reoxygenation myocardial cells was significantly higher than that of normal cultured cardiomyocytes (P<0.01). miR-7 mimics could enhance the expression level of miR-7 in myocardial cells, while miR-7 inhibitors could inhibit the expression of miR-7 in myocardial cells. miR-7 mimics could reduce the apoptosis rate of hypoxia reoxygenation myocardial cells and the expression of cleaved caspase-3 in the cells, inhibit the secretion of LDH in cardiac myocytes and promote the expression of p-Akt in the cells. miR-7 inhibitors could promote the apoptosis rate of hypoxia reoxygenation myocardial cells, increase the expression of cleaved caspase-3 in the cells, promote the secretion of LDH in cardiac myocytes and inhibit the expression of p-Akt in the cells. Conclusions The expression of miR-7 in myocardial hypoxia reoxygenation model is elevated. miR-7 can reduce the apoptosis of hypoxia reoxygenation myocardial cells, interference of miR-7 expression can promote the apoptosis of hypoxia reoxygenation myocardial cells, and the mechanism may be related to Akt signaling pathway.
费萍燕, 彭生, 费民忠, 陆海彬, 袁建英. miR-7在心肌细胞缺氧复氧模型中的表达及对心肌细胞凋亡的影响[J]. 实用预防医学, 2018, 25(2): 195-198.
FEI Ping-yan, PENG Sheng, FEI Min-zhong, LU Hai-bin, YUAN Jian-ying. Expression of miR-7 in myocardial hypoxia reoxygenation model and its effect on apoptosis of cardiomyocytes. , 2018, 25(2): 195-198.
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2018, Vol. 25 
