Abstract：Objective To explore the impact of nuclear factor-κB p65 (NF-κB p65) on the apoptosis of alveolar epithelial cells induced by Streptococcus pneumoniae and the expression of inflammatory cytokines. Methods Alveolar epithelial cells were divided into the control group, the infection group, the interference group and the negative control (NC) group. The infection group, the interference group and the NC group were treated with Streptococcus pneumoniae. The interference group and the NC group were transfected with siRNA NF-κB p65 and siRNA control respectively, while the control group was not treated. ELISA assay was used to detect the levels of IL-10 and IL-6 in cell culture supernatant, flow cytometry to detect cell apoptosis, RT-PCR to detect NF-κB p65 level in cells, and Western blot to detect Cleaved Caspase-3 and Bcl-2-associated X protein (Bax) levels in cells. Results The levels of NF-κB p65, IL-6, Cleaved Caspase-3 and Bax and the cell apoptosis rate were all significantly higher in the infection group and the NC group than in the control group, while the level of IL-10 was significantly lower in the above-mentioned two groups than in the control group (P<0.01). Compared with the infection group, the levels of NF-κB p65, IL-6, Cleaved Caspase-3 and Bax and the cell apoptosis rate were all significantly lower in the interference group (all P<0.01), while the level of IL-10 was significantly higher in the interference group (P<0.01). Conclusions NF-κB p65 can inhibit the apoptosis of alveolar epithelial cells induced by Streptococcus pneumoniae and the expression of inflammatory cytokines, and its mechanism may be related to the levels of Cleaved Caspase-3 and Bax .
王勇, 孙朝峰, 胡钱红. NF-κBp65对肺炎链球菌诱导的肺泡上皮细胞凋亡及炎性因子表达的影响[J]. 实用预防医学, 2018, 25(5): 547-550.
WANG Yong, SUN Zhao-feng, HU Qian-hong. Effect of NF-κB p65 on apoptosis of alveolar epithelial cells induced by Streptococcus pneumoniae and expression of inflammatory cytokines. , 2018, 25(5): 547-550.
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2018, Vol. 25 
