Abstract：Objective To explore the effect of RNA interference of high mobility group A1 (HMGA1) gene expression on the proliferation and apoptosis of glioma cells and its mechanism. Methods The mRNA and protein expression of HMGA1 in glioma tissue was detected by reverse transcription-polymerase chain reaction (RT-PCR) assay and Western blot. siRNA-NC, HMGA1-siRNA1 and HMGA1-siRNA2 were transfected into human glioma U251 cells, and the cells without any siRNA transfection served as the blank control group. After transfection for 48 hours, HMGA1 protein expression was detected. Cell proliferation was determined by CCK8 assay, cell apoptosis by flow cytometry, and Ki67, PCNA, cleaved caspase-3, β-catenin and cyclin D1 protein expression by Western blot. Results The mRNA and protein expression of HMGA1 gene in glioma tissue was significantly higher than that in the tumor-adjacent tissue (both P<0.01). RNA interference could significantly inhibit the expression of HMGA1 gene, and the inhibitory effect in HMGA1-siRNA2 group was more obvious, which was selected as the follow-up research object. As compared with the control group and siRNA-NC group, the cell survival rate and Ki67, PCNA, β-catenin and cyclin D1 protein expression in HMGA1-siRNA group were significantly decreased, while the cell apoptosis rate and cleaved caspase-3 protein expression were significantly increased (both P<0.01). Conclusions HMGA1 gene is highly expressed in glioma tissue, and inhibiting its expression can block the proliferation of human glioma U251 cell and promote its apoptosis by down regulation of Wnt/β-catenin signaling pathway.
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2018, Vol. 25 
