Epidemic analysis of varicella outbreaks and genotype of varicella-zoster virus in Shenzhen City, 2013-2017
LI Yuan1, YANG Gui-qing2, ZHANG Zhen1, GAO Shi-tong1, TANG Xiu-juan1, KONG Dong-feng1, QIN Yan-min1, MEI Shu-jiang1
1. Shenzhen Center for Disease Control and Prevention, Shenzhen, Guangdong 518055, China; 2. Luohu District Center for Disease Control and Prevention, Shenzhen, Guangdong 518020, China
Abstract:Objective To understand the epidemiological characteristics of varicella outbreaks and the genotype of varicella-zoster virus (VZV) in Shenzhen City, to explore the causes of high incidence of varicella on the basis of genotyping, and to provide a scientific basis for prevention and control of varicella. Methods Descriptive epidemiological approaches were applied to analyze the data regarding varicella outbreaks in Shenzhen from 2013 to 2017. Blister specimens were collected from some patients clinically diagnosed with varicella, and DNA was extracted from these specimens. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay was performed to identify strains, and sequencing comparison to analyze their genotypes. Results A total of 579 outbreaks of varicella were reported in Shenzhen City from 2013 to 2017. There were two peaks of the outbreaks, one peak in March-June and another from November to January of the following year. Most outbreaks were reported in Longgang District (31.43%), Luohu District (17.27%) and Nanshan District (11.40%). The majority of the outbreaks occurred in primary and secondary schools and kindergartens. The longest duration of the outbreaks was 120 days. Nucleic acids of VZV were detected in 17 patients, and all VZV strains belonged to J genotype. Conclusions The epidemics of varicella in Shenzhen City during 2013-2017 were severe, and the genotype of prevalent VZV strains was J. It is necessary to implement the two-dose varicella vaccination program in the susceptible population so as to prevent and control its epidemics.
[1] 庞红, 王晨, 汤素文,等. 上海市某小学水痘暴发调查及应急接种的血清学评价[J]. 现代预防医学,2013, 40(14):2730-2732,2741. [2] 王维治. 神经病学[M]. 北京: 人民卫生出版社, 2006:740. [3] Bamford J, Sandercock P, Dennis M, et al. Classification and natural history of clinically identifiable subtypes of cerebral infarction[J]. Lancet, 1991, 337 (8756):1521-1526. [4] 王创新,李全霞,赵云,等. 2006-2013年济南市历城区水痘疫情流行病学分析[J]. 现代预防医学, 2015, 42(1):157-159. [5] 崔伟红,姜梅,李娜,等. 2008-2014年烟台市学校水痘流行特征分析[J]. 现代预防医学, 2015, 42(19):3581-3583, 3605. [6] 杨琼,张星. 2012-2014年成都市金牛区水痘流行病学特征及免疫策略分析[J]. 寄生虫病与感染性疾病,2016, 14(4):261-263. [7] 殷大鹏,宋立志,张晓曙,等. 鲁甘湘三省2007年水痘发病率调查[J]. 中国疫苗和免疫,2009,15(2):119-122. [8] 唐文钊,丘文清,朱秀兰,等. 惠州市2011-2015年水痘疫情流行特征与疫苗接种情况分析[J]. 实用预防医学,2018,25(1):102-104. [9] 许青,徐爱强. 我国水痘流行病学分析与水痘疫苗免疫预防研究进展[J]. 中国新药杂志, 2012, 21(10):1093-1098. [10] 杨洁,黄芳,林喜乐. 水痘减毒活疫苗两针法免疫效果及安全性评价[J]. 现代预防医学,2015,42(14):2630-2632. [11] Lu L, Suo L, Li J, et al. A varicella outbreak in a school with high one-dose vaccination coverage, Beijing, China[J]. Vaccine, 2012,30(34):5094-5098. [12] 何蔚云,梁建华,吴莉萍,等. 儿童集体单位水痘暴发与水痘疫苗接种效果的病例对照研究[J]. 中国学校卫生, 2013,34(4):465-467. [13] 杨立清,李丽,张楠. 水痘疫苗2剂接种程序对北京市朝阳区水痘发病的影响[J]. 中国生物制品学杂志, 2015,28(11):1177-1182. [14] 旷翠萍,林喜乐,庄辉元,等. 一起学校水痘暴发疫情的流行因素及疫苗保护效果调查[J]. 实用预防医学,2017,24(6):699-701. [15] Loparev VN, Gonzalez A, Deleon-Carnes M, et al. Global identification of three major genotypes of varicella-zoster virus:longitudinal clustering and strategies for genotyping[J]. J Virol, 2004,78(15):8349-8358. [16] Sengupta N,Taha Y,Scott FT,et al. Varicella-zoster-virus genotypes in East London:a prospective study in patients with herpes zoster [J]. J Infect Dis,2007,196(7):1014-1020.