Abstract：Objective To explore the impact of fine particulate matter (PM2.5) on the expression of lung cancer-related lncRNAs in human bronchial epithelial cells (BEAS2B). Methods Normal BEAS2B cells treated with phosphate buffer saline (PBS) for 48 hours served as the control group, while BEAS2B cells treated with 300 μg/ml PM2.5 for 48 hours was considered as the treated group. The changes of expression of lncPVT1, lncUCA1, MIR31HG, lncIRAIN, lncCASC2, lncH19 and lncSNHG were detected by quantitative real-time polymerase chain reaction assay. Results The expression of lncPVT1, lncUCA1, MIR31HG and lncIRAIN in the treated group was up-regulated and increased by 0.93-fold, 0.45-fold, 2.80-fold and 2.94-fold respectively as compared with the control group, with statistically significant differences (P<0.05). And the expression of lncIRAIN was affected most significantly. The expression of lncCASC2and lncH19in the treated group was down-regulated and decreased by 0.17-fold and 0.08-fold respectively as compared with the control group, with statistically significant differences (P<0.05). And the expression of lncH19 was affected most significantly. Conclusions PM2.5 can enhance the expression of MIR31HG, lncPVT1, lncUCA1 and lncIRAIN, but inhibit the expression of lncCASC2 and lncH19.
[1] Thomson EM, Breznan D, Karthikeyan S, et al. Contrasting biological potency of particulate matter collected at sites impacted by distinct industrial sources[J]. Part Fibre Toxicol, 2016,13(1):65-82. [2] 杨露, 袁雅冬. PM2.5的氧化损伤机制及其与呼吸系统疾病关系[J]. 临床荟萃, 2016,31(4):433-438. [3] 杨财平, 马蓓蓓, 余青, 等.宜昌市PM2.5的污染特征及其风险评价[J]. 实用预防医学, 2017,24(10):1209-1212. [4] Engreitz JM, Ollikainen N, Guttman M. Long non-coding RNAs: spatial amplifiers that control nuclear structure and gene expression[J]. Nat Rev Mol Cell Biol, 2016,17(12):756-770. [5] Bouckenheimer J, Assou S, Riquier S, et al. Long non-coding RNAs in human early embryonic development and their potential in ART[J]. Hum Reprod Update, 2016,23(1):19-40. [6] 刘林华, 蒋义国, 纪卫东, 等. 结晶型硫化镍对人支气管上皮细胞PTEN基因和miR-22表达的影响[J]. 环境与健康杂志, 2010,27(5):393-395. [7] Deng X, Feng N, Zheng M, et al. PM2.5 exposure-induced autophagy is mediated by lncRNA loc146880 which also promotes the migration and invasion of lung cancer cells[J]. Biochimi Biophys Acta, 2017,1861(2):112-125. [8] Feng J, Sun Y, Zhang EB, et al. A novel long noncoding RNA IRAIN regulates cell proliferation in non small cell lung cancer[J]. Int J Clin Exp Pathol, 2015,8(10):12268-12275. [9] Wang P, Liu YH, Yao YL, et al. Long non-coding RNA CASC2 suppresses malignancy in human gliomas by miR-21[J]. Cell Signal, 2015,27(2):275-282. [10] Li P, Xue WJ, Feng Y, et al. Long non-coding RNA CASC2 suppresses the proliferation of gastric cancer cells by regulating the MAPK signaling pathway[J]. Am J Transl Res, 2016,8(8):3522-3529. [11] He X, Liu Z, Su J, et al. Low expression of long noncoding RNA CASC2 indicates a poor prognosis and regulates cell proliferation in non-small cell lung cancer[J]. Tumour Biol, 2016,37(7):9503-9510. [12] Montes M, Nielsen MM, Maglieri G, et al. The lncRNA MIR31HG regulates p16(INK4A) expression to modulate senescence[J]. Nat Commun, 2015,6:6967. [13] Cui J, Mo J, Luo M, et al. c-Myc-activated long non-coding RNA H19 downregulates miR-107 and promotes cell cycle progression of non-small cell lung cancer[J]. Int J Clin Exp Pathol, 2015,8(10):12400-12409. [14] Wan L, Sun M, Liu GJ, et al. Long noncoding RNA PVT1 promotes non-small cell lung cancer cell proliferation through epigenetically regulating LATS2 expression[J]. Mol Cancer Ther, 2016,15(5):1082-1094. [15] Cui D, Yu CH, Liu M, et al. Long non-coding RNA PVT1 as a novel biomarker for diagnosis and prognosis of non-small cell lung cancer[J]. Tumour Biol, 2016,37(3):4127-4134. [16] Wang HM, Lu JH, Chen WY, et al. Upregulated lncRNA-UCA1 contributes to progression of lung cancer and is closely related to clinical diagnosis as a predictive biomarker in plasma[J]. Int J Clin Exp Med, 2015,8(7):11824-11830.
2019, Vol. 26 
