In Vitro Induction of Erythromycin Resistant in Bartonella henselae
SONG Xiu-Ping, LIU Qi-Yong, LI Dong-Mei*
WHO Collaborating Center for Vector Surveillance and Management,Department of Vector Biology and Control, State Key Laboratory for Infectious Diseases Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Abstract:[Objective] Erythromycin has been often used to treat Bartonella-associated infections. Our aim here was to induction of erythromycin resistant in B. henselae. [Methods] The reference strain Houston-1(ATCC 49882)and the six wild strains were selected as the candidates for the induction of erythromycin resistant, which were all susceptible to erythromycin. The original strains, which were subcultured three passages after recovery were used to induce drug resistance. The initiative concentration of erythromycin was 1/4 × MIC. In vitro induction was carried out on serial doubling concentrations of antibiotics incorporated into agar.MICs for erythromycin and the other antibiotics were determined using the agar dilution and E-test methods. The blank control of B. henselae strain was measured MICs for detecting whether subcultrues have an effect on the development of resistance. [Results] After 12 passages on antibiotics plates, six strains acquired resistance to erythromycin and azithromycin with MICs > 256 mg/L. The six erythromycin-resistant mutants also displayed resistant to clindamycin. All were sensitive to other antibiotics. After 30 passages on plates, four strains had a certain level of resistance to clindamycin,the others had not changed. [Conclusion] Bartonella henselae can be induced to be resistant to erythromycin and azithromycin in vitro by increasing drug concentration serially and the acquired erythromycin-resistant strains were resistant to clindamycin. The study provided a model of fastidious, facultative intracellular bacilli for the further study of mechanism of Bartonella resistance to macrolides.
宋秀平,刘起勇,刘云彦,栗冬梅. 汉赛巴尔通体红霉素体外诱导耐药研究[J]. 实用预防医学, 2015, 22(6): 645-647.
SONG Xiu-Ping, LIU Qi-Yong, LI Dong-Mei. In Vitro Induction of Erythromycin Resistant in Bartonella henselae. , 2015, 22(6): 645-647.