Abstract：Objective To study the molecular epidemiology of norovirus causing an outbreak of acute gastroenteritis. Methods Specimens were collected from patients with acute gastroenteritis in a tour group in Beijing on February 7, 2015, and were subjected to norovirus detection by real-time polymerase chain reaction (real-time PCR). Gene detection and analysis of both RNA-dependent RNA polymerase (RdRp) and VP1 region were performed in the noroviruses isolated. Results Twelve out of 18 stool specimens were detected to be GⅡ norovirus-positive by real-time PCR. And 11 norovirus sequences were obtained by gene amplification of both RdRp and VP1 region. Phylogenetic analysis of RdRp and VP1 region indicated that norovirus GⅡ.17 was the pathogen. The virus strain was highly homologous with Zhuhai ZHITHC-12 strain, which had caused outbreaks of infectious diseases before. The nucleotide difference was 0% between the laboratory strain and Zhuhai ZHITHC-12 strain in the RdRp region, and 0.1% in the VP1 region. The amino acid differences were both 0%. Conclusions It is a new norovirus variant of GⅡ.17 causing outbreaks of acute gastroenteritis in China in 2015 thatleads to the outbreak of the acute gastroenteritis in a tour group in Beijing.
张爽， 荆红波， 马红梅， 李颖， 靳淼. 一起急性胃肠炎暴发疫情中诺如病毒的分子流行病学分析[J]. 实用预防医学, 2017, 24(1): 15-18.
ZHANG Shuang, JING Hong-bo, MA Hong-mei, LI Ying, JIN Miao. Molecular epidemiology of norovirus in an outbreak of acute gastroenteritis. , 2017, 24(1): 15-18.
2017, Vol. 24 
