Abstract：ObjectiveTo establish a fast colloidal gold diagnostic assay for Streptococcus suis Ⅱ infection. MethodsThe conservative protein GDH encoding gene gdh was target gene for cloning, expression and purification. A rapid colloidal gold diagnostic assay for Streptococcus suis Ⅱ infection was established by using microporous membrane carrier such as nitrocellulose membrane and so on to envelope purified protein, using gold granule to label SPA. ResultsColloidal gold test strip appeared on the positive results of specimens of two red bands, the negative results of only a quality control line strip near the suction end is one red band. ConclusionsUse of colloidal gold immunochromatography can rapidly diagnose Streptococcus suis serotype 2 infection.
胡玉山,肖丽红,刘巧谊,候水平,刘俊华,周勇,吴新伟,杨智聪. 猪链球菌2型感染胶体金诊断方法的研究[J]. 实用预防医学, 2015, 22(6): 675-677.
HU Yu-shan, XIAO Li-hong, LIU Qiao-yi, HOU Shui-ping, LIU Jun-hua, ZhOU Yong, WU Xing-wei, YANG Zhi-chong. Study on colloidal gold rapid diagnosis for streptococcus suis serotype 2 infection. , 2015, 22(6): 675-677.
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