Abstract：ObjectiveTo identify whether there were infections with vaccine strains of measles virus in Changsha in 2013- 2014 and to establish laboratory methods to rapidly distinguish wild-type and vaccine strains of measles virus. Methods According to National Measles Surveillance Programme, throat swab specimens were collected from suspected measles patients and nucleic acid of measles virus was detected by real-time PCR (RT-PCR). Then, rapid identification and gene sequence analysis of wild-type and vaccine strains of measles virus in positive samples were performed by reverse transcription-polymerase chain reaction-restriction fragment length polymorphism (RT-PCR-RFLP) after RNA was extracted. Result Totally, 224 positive specimens were detected in 697 samples, and 9 patients with measels clinical manifestations had been vaccinated with measles attenuated live vaccines. RT-PCR-RFLP revealed that all of the cases were infected with wild-type measles strains. Genotype sequencing and sequence alignment analysis indicated that the genotypes of the isolates were all H1a. Conclusions The suspected measles patients and nine measles patients with immunization of measles attenuated live vaccines in Changsha in 2013-2014 were all infected with wild-type measles virus. Rapid identification of measles vaccine and wild-type strains is established.
2016, Vol. 23 
