Determination of serum dopamine content based on fluorescence quenching probe of nitrogen-boron-doped carbon quantum dots
BAI Zuhai1, YAN Zhihong2, XU Mingzhong1
1. Changsha Municipal Center for Disease Control and Prevention, Changsha, Hunan 410001, China; 2. Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, China
Abstract：Objective To establish a method for measuring dopamine (DA) content in serum by nitrogen-boron-doped carbon quantum dots (N, B-CQDs) as a fluorescence probe. Methods N, B-CQDsb was prepared by hydrothermal method with sodium citrate as carbon source, 2-amino-3-hydroxypyridine as nitrogen source, and sodium borohydride as boron source. Transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS) and fourier transform infrared spectrometer (FTIR) were used to characterize N, B-CQDs. In pH 9.0 sodium carbonate-sodium bicarbonate buffer solution, DA and N,B-CQDs were heated in a constant temperature water bath at 40℃ for 200 min, the excitation wave length was 354 nm and the emission wave length 420 nm. DA had quenching effect on fluorescence of N, B-CQDs, and the quantitative analysis of DA could be realized. Results Under optimal experimental conditions, the fluorescence quenching of N, B-CQDs by DA showed good linear relationships with the concentration of DA in the range of 5×10-6mol/L-1.50×10-4mol/L, with the detection limit of 1.3×10-6mol/L. The method was successfully applied to determination of DA content in serum, and the recoveries were between 99.24% and 102.3%. The relative standard deviations were in the range of 1.8%-3.1%. Conclusion A method for DA analysis and detection based on fluorescence quenching of N, B-CQDs is constructed. And the proposed method is simple operation, higher sensitivity and better selectivity.
2023, Vol. 30 
