Abstract：Objective To understand the disinfection performance of a hydrogen peroxide low-temperature surface disinfectant, and to provide a scientific basis for surface disinfection of low-temperature objects. Methods At the temperature of -20.1℃ -22.6℃, the quantitative killing test of bacterial carriers, virus inactivation test and simulated field disinfection test in low-temperature freezer were performed, and the physicochemical features and acute oral toxicity (LD50) were determined simultaneously. Results The disinfectant had stable performance when its pH was 5.25 and the main bactericidal component was 1.91% hydrogen peroxide. It had mild corrosion to stainless steel, copper and aluminum, and moderate corrosion to carbon steel. Under the condition of -20.1℃-22.6℃, the disinfectant did not freeze for 5 days, and the killing logarithmic values ofEscherichia coli for a contact time of 5 minutes, Staphylococcus aureus for a contact time of 10 minutes, and poliovirus type I (PV-I) vaccine strain for a contact time of 20 minutes were all greater than 3.00. After 30 simulated field tests, the stock solution of this disinfectant killed Staphylococcus aureus for a contact time of 20 minutes, and the killing logarithmic values were all greater than 3.00. The LD50 was more than 5,000 mg/(kg.bw), which belonged to the actual non-toxic level. It had no irritation to the skin of white rabbits for many times, and the micronucleus test of polychromatic erythrocytes in mouse bone marrow was negative. Conclusion The properties of the disinfectant can meet the disinfection requirements of killing Escherichia coli and Staphylococcus aureus. The disinfectant can effectively inactivate poliovirus type I (PV-I) vaccine strain, and is non-toxic and non-irritating to the skin. It can be widely used for surface disinfection of low-temperature objects and effectively cut off the route of contact and transmission of cold-chain food contaminated with SARS-CoV-2.
2023, Vol. 30 
