Abstract：Objective To understand the efficacy of an ethanol compound disinfectant in killing bacteria,Candida albicans and viruses, and to provide a scientific basis for selecting qualified and effective hand disinfectants for hand hygiene disinfection. Methods Physical and chemical detection, carrier quantitative sterilization test, virus inactivation test and hand and skin disinfection field test were performed to assess the antisepsis effect of an ethanol compound disinfectant. Results The pH value of the disinfectant was 7.49, the original solution contained 71.7% (v/v) ethanol and 0.42% hydrogen peroxide. The decline rate of the active ingredient content met the requirements of the virus inactivation stability test. After the effect time of 0.5min, the logarithmic values of killing Staphylococcus aureus ATCC6538, Escherichia coli 8099 and Pseudomonas aeruginosa ATCC15442 were all greater than 5.0, and the logarithmic values of killing Candida albicans ATCC10231 were all greater than 4.0. After the effect time of 0.5min, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and H1N1 (WSN) influenza viruses could be effectively inactivated. After the effect time of 1.0min, the poliovirus vaccine strain (PV-1) could be effectively inactivated. Avian influenza virus had strong resistance, and its inactivation time needed to be extended to 3 minutes. After 30 field tests of hand and skin disinfection, the logarithmic mean values of killing natural bacteria on hands and skin for 1.0min were 2.55 (1.36-2.91) and 2.50 (1.67-2.84) respectively. Conclusion The disinfectant has stable performance, and can quickly kill bacteria and Candida albicans and effectively inactivate H1N1(WSN) influenza virus, SARS-CoV-2, poliovirus and avian influenza virus. It can be widely used for hand and skin disinfection, and reduce the incidence of infections.
2023, Vol. 30 
