Abstract：Objective To explore the effect of perinatal exposure to perfluorooctane sulfonate (PFOS) on key gene expression of BDNF/TrkB/CREB signaling pathways in hippocampus of mice offspring. Methods Twenty pregnant Kunming mice were randomly divided into the control group and the low-, middle- and high-dose groups. The mice in the low-, middle- and high-dose groups were respectively administrated with 0.1, 1.0 and 5.0 mg/(kg·bw) PFOS by gavage from the gestation day 2 to the postnatal day 21. The control group received 0.05% Tween-20 solution. The gavage volume was 0.1 ml/10 (g·bw). The offspring’s brain tissues were collected and observed pathologically by hematoxylin-eosin (HE) staining on the postnatal day 21. Quantitative real-time PCR (QPCR) was used to detect mRNA expression levels of brain-derived neurotrophic factor (BDNF), tropomyosin-related receptor kinase B (TrkB), cAMP response element-binding protein (CREB), synapsin1 (Syn1) and synaptophysin (Syp) in hippocampus tissues. Results No statistically significant differences were found in the mortality rate and body weight of mice offspring between the control group and the low-, middle- and high-dose groups (P>0.05). However, vacuole was observed in offspring’s hippocampus tissues in the high-dose PFOS group. The mRNA levels of BDNF, TrkB and CREB in hippocampus tissues significantly decreased from (0.98±0.11), (1.03±0.09) and (1.08±0.12) in the control group to (0.22±0.21), (0.71±0.14) and (0.37±0.26), respectively. The mRNA levels of Syn1 and Syp in the middle- and high-dose PFOS groups also significantly decreased, which decreased from (1.10±0.09) and (0.97±0.08) in the control group to (0.41±0.23) and (0.71±0.17) in the middle-dose PFOS group and (0.39±0.19) as well as (0.63±0.19) in the high-dose PFOS group, showing statistically significant differences (all P<0.05). Conclusions PFOS can damage BDNF/TrkB/CREB signaling pathways in hippocampus tissues, which may be one of the mechanisms leading to developmental neurotoxicity of PFOS.
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2019, Vol. 26 
