人参皂苷Rb1对周围神经损伤后大鼠背根神经节神经生长因子及其受体酪氨酸激酶A表达的影响

doi:10.3969/j.issn.1006-3110.2019.04.008

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摘要目的观察人参皂苷Rb1对周围神经损伤后大鼠背根神经节神经生长因子(nerve growth factor, NGF)及其受体酪氨酸激酶A(tyrosine kinase A,TrkA)表达的影响。方法将SD大鼠随机分为5组:假手术组、模型组、甲钴胺组(10.8 mg/kg)、人参皂苷Rb1低剂量组(25 mg/kg)、人参皂苷Rb1高剂量组(50 mg/kg)。采用神经夹持损伤法建立周围神经损伤模型,造模完成后灌胃给药,持续14 d。末次给药后,采用光热耐痛阈测定法评价大鼠的感觉恢复情况,Western-blotting法检测大鼠背根神经节NGF及其受体TrkA蛋白表达情况,RT-PCR法检测基因表达情况。结果与假手术组比较,模型组大鼠对热痛感觉的灵敏度下降(11.23±2.11 vs. 7.14±0.67,P<0.01),背根神经节NGF及其受体TrkA蛋白和基因表达均显著下调(0.34±0.05 vs. 0.87±0.11,0.31±0.04 vs. 0.79±0.10,P<0.01);给予高剂量的人参皂苷Rb1干预后,模型大鼠的热痛感觉恢复良好,数值为(8.36±0.92)(P<0.01),背根神经节NGF、TrkA的蛋白和基因水平均得到回升,分别为(0.65±0.07)、(0.70±0.08)(P<0.01)。结论人参皂苷Rb1能通过提高背根神经节NGF及其受体TrkA的表达,维持神经元存活,促进受损神经修复,改善周围神经损伤后大鼠的感觉功能。

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	邓明
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	陈庆
	刘世清
	吴飞
	明江华

关键词 ：周围神经损伤, 人参皂苷Rb1, 背根神经节, 神经生长因子, 酪氨酸激酶A

Abstract：Objective To observe the influence of ginsenoside Rb1 on nerve growth factor (NGF) and its receptor tyrosine kinase A (TrkA) expression in dorsal root ganglion in rats with peripheral nerve injury. Methods SD rats were randomly divided into the sham operation group, the model group, the methylcobalamin group (10.8 mg/kg) and the low-dose (25 mg/kg) and high-dose (50 mg/kg) ginsenoside Rb1 groups. Nerve clamping injury method was used to establish the peripheral nerve injury models, and then the model rats were administered intragastrically for 14 successive days. After the last dose, the sensory recovery of the rats was assessed using the photothermal pain threshold method. The expression of NGF and its receptor TrkA in rat dorsal root ganglion was detected by Western-blotting, and the gene expression was detected by reverse transcription polymerase chain reaction (RT-PCR). Results Compared with the sham operation group, the sensitivity of thermal sensation in the model group was decreased (11.23±2.11 vs. 7.14±0.67, P<0.01), and the expression of NGF and its receptor TrkA protein and gene in dorsal root ganglion were significantly down-regulated (0.34±0.05 vs. 0.87±0.11, 0.31±0.04 vs. 0.79±0.10, both P<0.01). After intervention of high-dose ginsenoside Rb1, the thermal pain of the model rats recovered well (P<0.01), with the value of (8.36±0.92); and the protein and gene levels of NGF (0.65±0.07) and TrkA (0.70±0.08) in dorsal root ganglion recovered (both P<0.01). Conclusions Ginsenoside Rb1 can increase the expression of NGF and its receptor TrkA in dorsal root ganglion, maintain the survival of neurons, promote the repair and regeneration of damaged nerves, and improve the sensory function of rats after peripheral nerve injury.

Key words： peripheral nerve injury ginsenoside Rb1 dorsal root ganglion nerve growth factor tyrosine kinase A

收稿日期: 2018-05-10

R-332

基金资助:国家自然科学青年基金项目(81301052);中央高校基本科研业务费专项基金(2042017kf0139)

通讯作者: 明江华,E-mail:mingjianghua2002@medmail.com.cn。

作者简介: 邓明(1983-),男,博士,主治医师,研究方向:神经再生。

引用本文:

邓明, 谢萍, 马永刚, 刘志勇, 周炎, 陈庆, 刘世清, 吴飞, 明江华. 人参皂苷Rb1对周围神经损伤后大鼠背根神经节神经生长因子及其受体酪氨酸激酶A表达的影响[J]. 实用预防医学, 2019, 26(4): 412-415. DENG Ming, XIE Ping, MA Yong-gang, LIU Zhi-yong, ZHOU Yan, CHEN Qing, LIU Shi-qing, WU Fei, MING Jiang-hua. Effects of ginsenoside Rb1 on the expression of nerve growth factor and its receptortyrosine kinase A in rat dorsal root ganglion after peripheral nerve injury. , 2019, 26(4): 412-415.

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