Abstract：Objective To study the therapeutic effect of recombination human keratinocyte growth factor-2 (rhKGF-2) on experimental corneal alkaline burn in rats. Methods Corneal injuries were induced by alkaline burn with 1 mol/L NaOH solution in 70 female SD rats. The rats were then randomly divided into the model control group, the recombinant bovine basic fibroblast growth factor in eye drop (bFGF) group and the low-, middle- and high-dose rhKGF-2groups (each n=14). The bFGF group was treated with 4,500 IU/mL bFGF, and the low-, middle- and high-dose rhKGF-2 groups were respectively treated with 1.25 μg/ml, 25 μg/ml and 50 μg/ml rhKGF-2, 50 μl per time, 3 times a day for 14 days. The corneal injury grades and the healing rate of corneal endothelium were measured on the 1st, 3rd, 7th and 14th days after the start of treatment. Body weight and corneal histopathologic changes were examined on the 7th and 14th days after the start of treatment. Results No statistically significant differences were found in body weight and corneal injury degree among all groups on each time points (P>0.05). Corneal thickness of the rats in the bFGF group decreased on the 14th day after the start of treatment (P<0.05). Corneal endothelium healing rate of the rats in the rhKGF-2 groups increased on the 3rd and 7th days after the start of treatment (P<0.05), whereas their corneal thickness decreased on the 14th day after the start of treatment (P<0.05). Compared with the rats in the bFGF group, corneal endothelium healing rate of the high-dose rhKGF-2 group increased on the 3rd day after the start of treatment (P<0.05). Conclusions rhKGF-2 is effective on experimental corneal alkaline burn in rats, especially in the early treatment.
2018, Vol. 25 
