脓毒症小鼠血清HMGB1与心肌损伤程度、炎症相关因子、BNP及cTnI水平的关系

doi:10.3969/j.issn.1006-3110.2019.04.017

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摘要目的探讨血清高迁移率族蛋白B1(high mobility group box 1,HMGB1)在脓毒症小鼠心肌损伤中的作用及与心肌细胞炎症因子、血清B型钠尿肽(B-type natriuretic peptide,BNP)和肌钙蛋白(troponin,cTnI)的相关性。方法本研究选取6～8周龄、SPF级的昆明系小鼠60只,采用随机数字表法分为模型组、假手术组各30只,采用小鼠盲肠结扎穿孔法建立脓毒症小鼠模型,观察不同时间点小鼠血清HMGB1的水平变化,探讨其与心肌细胞凋亡率、心肌细胞炎症因子、血清B型钠尿肽(BNP)和肌钙蛋白(cTnI)的相关性。结果在0 h时刻,模型组和假手术组的血清HMGB1水平、心肌细胞凋亡率差异无统计学意义(P>0.05);在造模后12、24、48及72 h,模型组小鼠的血清HMGB1水平、心肌细胞凋亡率显著高于假手术组,差异均有统计学意义(P<0.05);在造模后48 h,模型组心肌组织中肿瘤坏死因子(tumor necrosis factor-α,TNF-α)、白细胞介素-6(interleukin-6,IL-6)、IL-10、血清BNP、血清cTnI水平显著高于假手术组(P<0.05);在造模后48 h,模型组心肌组织中TNF-α、IL-6、IL-10、血清BNP、血清cTnI水平与血清HMGB1均呈显著正相关(P<0.05)。结论血清HMGB1在脓毒症小鼠中水平升高趋势明显,并且与小鼠心肌损伤具有密切的关系。

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	韩延辉
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	贾静静
	董小伟

关键词 ：脓毒症, 小鼠, 心肌损伤, 炎症因子, B型钠尿肽, 肌钙蛋白

Abstract：Objective To explore the role of serum high mobility group box 1 (HMGB1) in myocardial injury in septic mice and its correlations with myocardial inflammatory factors, serum B-type natriuretic peptide (BNP) and troponin (cTnI). Methods Sixty Kunming mice with 6-8 weeks old and SPF grades were selected in this study, and then the random number table method was used to divide them into the model group and the sham operation group (each n=30). Sepsis models were established by cecal ligation and puncture in mice. The changes of serum HMGB1 levels in mice at different time points were observed, and then its correlations with cardiomyocyte apoptosis rate (CMAR), myocardial inflammatory factors, serum BNP and cTnI were explored. Results No statistically significant differences were found in serum HMGB1 level and CMAR at 0 h between the model group and the sham operation group (both P>0.05). At 12, 24, 48 and 72 hours after the model establishment, serum HMGB1 level and CMAR were significantly higher in the model group than in the sham operation group, with statistically significant differences (both P<0.05). At 48 hours after the model establishment, the levels of tumor necrosis factor-α (TNF-α),interleukin-6 (IL-6), interleukin-10 (IL-10), serum BNP and serum cTnI were significantly higher in the model group than in the sham operation group (all P<0.05). At 48 hours after the model establishment, the levels of TNF-α, IL-6, IL-10, serum BNP and serum cTnI in the myocardial tissue of the model group were significantly positively correlated with serum HMGB1 (all P<0.05). Conclusions Serum HMGB1 level shows a significant increasing tendency in septic mice, and is closely related to myocardial damage in mice.

Key words： sepsis mouse myocardial injury inflammatory factor B-type natriuretic peptide troponin

收稿日期: 2018-02-19

R-332

作者简介: 韩延辉(1978-),男,河南洛阳人,硕士,副主任医师,研究方向:冠心病及心律失常的基础与临床。

引用本文:

韩延辉, 王燕, 王红雷, 贾静静, 董小伟. 脓毒症小鼠血清HMGB1与心肌损伤程度、炎症相关因子、BNP及cTnI水平的关系[J]. 实用预防医学, 2019, 26(4): 450-452. HAN Yan-hui, WANG Yan, WANG Hong-lei, JIA Jing-jing, DONG Xiao-wei. Relationship of serum high mobility group box 1 with myocardial injury, inflammatory related factors and changes of B-type natriuretic peptide and troponin in septic mice. , 2019, 26(4): 450-452.

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[1] 顾忠民, 林绍彬, 刘才俊,等. 桃红芩连汤对脓毒症大鼠心肌高迁移率族蛋白B1的影响[J]. 中国中西医结合杂志, 2016, 36(4):445-448.
[2] 唐关敏, 翟昌林, 胡惠林,等. 针灸预处理对大鼠心肌缺血/再灌注后高迁移率族蛋白B1的影响[J]. 中国中西医结合急救杂志, 2015,13(1):33-37.
[3] 冯宣云, 艾山木, 刘琼. HMGB1在脓毒症心肌损害小鼠中的表达及炎症促进作用研究[J]. 重庆医科大学学报, 2017,13(5):509-514.
[4] 曾鸣, 张玉坤, 吴永昌,等. 苦柯胺B通过抑制高迁移率族蛋白B1/核转录因子-κB信号通路减轻脓毒症小鼠肺损伤[J]. 中华危重病急救医学, 2016, 28(11):994-997.
[5] 陈勉, 胡志华, 陈伟. 持续血液净化疗法治疗外伤继发性脓毒症临床效果及对高迁移率族蛋白1、肿瘤坏死因子-α和促血管生成素2的影响[J]. 中国现代医学杂志, 2014, 24(1):61-64.
[6] Masson S, Caironi P, Fanizza C, et al. Sequential N-terminal pro-B-type natriuretic peptide and high-sensitivity cardiac troponin measurements during albumin replacement in patients with severe sepsis or septic shock[J].Crit Care Med, 2016, 44(4):707-716.
[7] 张轶庠, 袁也晴, 魏育英,等. 高迁移率族蛋白-1在尿源性脓毒血症家兔肾脏组织中的表达及意义[J]. 中华实验外科杂志, 2015, 32(6):1264-1266.
[8] Lurati Buse GA, Bolliger D, Seeberger E, et al. Troponin T and B-type natriuretic peptide after on-pump cardiac surgery: prognostic impact on 12-month mortality and major cardiac events after adjustment for postoperative complications[J]. Circulation, 2014, 130(12):948-957.
[9] 高鹏飞, 张锋, 程彬彬,等. 核因子-κB介导脂多糖诱导急性肺损伤大鼠高迁移率族蛋白B1的表达[J]. 临床麻醉学杂志, 2015, 31(3):278-281.
[10] Zhang C, Liu R, Yuan J, et al. Predictive values of N-terminal pro-B-type natriuretic peptide and cardiac troponin I for myocardial fibrosis in hypertrophic obstructive cardiomyopathy[J]. PLoS One, 2016, 11(1):e0146572.
[11] 邱英, 郑世翔, 丁国华,等. 高迁移率族蛋白1对脓毒症及相关急性肾损伤的诊断和预后评估价值研究[J]. 中国全科医学, 2016, 19(8):886-893.
[12] Gaggin HK, Dang PV, Do LD, et al. Reference interval evaluation of high-sensitivity troponin T and N-terminal B-type natriuretic peptide in Vietnam and the US: The North South East West Trial[J]. Clin Chem, 2014, 60(5):758-764.
[13] 魏佳鑫, 张莹, 马晓媛,等. 警报素高迁移率族蛋白B1在脓毒症中的作用[J]. 中华危重病急救医学, 2016, 28(8):761-764.
[14] 李徽徽, 仇大鹏, 高琴,等. 选择性激动黑皮质素4型受体(MC4R)通过HMGB1/TLR4/NF-κB信号途径对抗大鼠脓毒症致急性肝损伤[J]. 细胞与分子免疫学杂志, 2016, 32(8):1055-1059.
[15] Srivastava PK, Pradhan AD, Cook NR, et al. Impact of modifiable risk factors on B-type natriuretic peptide and cardiac troponin T concentrations[J]. Am J Cardiol, 2016, 117(3):376-381.