Abstract：Objective To analyze the results of pathogenic surveillance of scarlet fever in Changping District of Beijing in 2016. Methods Quantitative polymerase chain reaction (qPCR) assay and plate scribing isolation technique were respectively used to detect the positive rates of carrying group A streptococcus(GAS) of the cases, and the isolated GAS strains were typed by emm genotyping. The identification of the isolated non-GAS strains was conducted using direct 16S rRNA gene sequencing and VITEK2 Compact automatic microbe instrument. Results Among the 132 throat swab specimens collected from the cases in Changping District in 2016, 13 GAS strains were detected by plate scribing and 19 non-GAS strains by qPCR, with the positive rates being 9.85% and 14.39% respectively. The isolated GAS strains were proved to be emm1 and emm12 genotypes. 63 non-GAS strains were isolated, and then identified to belong to 5 genera and 13 species. Conclusions The emm genotypes in Changping District in 2016 were far less diversified. qPCR assay, which has a higher positive detection rate of GAS, is more sensitive than plate scribing isolation method. VITEK2 Compact automatic microbe instrument analysis has obvious limitations in the identification of the non-GAS strains as compared with direct 16S rRNA gene sequencing, and the positive rates detected by the two methods do not match well.
吴杨,赵炳岩,高进玺,张阳,庄鹏,李东迅,舒高林. 2016年北京市昌平区A组乙型溶血性链球菌及相关菌株检测和分析[J]. 实用预防医学, 2018, 25(4): 448-451.
WU Yang, ZHAO Bing-yan,GAO Jin-xi, ZHANG Yang, ZHUANG Peng, LI Dong-xun, SHU Gao-lin. Detection of group A streptococcus and the relevant strains in Changping District of Beijing, 2016. , 2018, 25(4): 448-451.
2018, Vol. 25 
