Abstract：Objective To explore the neurotoxicity of beta-cypermethrin and to provide a theoretical basis for its poisoning prevention and treatment. Methods Eighty healthy adult Kunming mice were randomly divided into four groups, with 20 mice in each group (10 males and 10 females). Three experimental groups were respectively administered with 20, 40 and 80 mg/kg beta-cypermethrin dissolved in edible oil by a single oral gavage. The control group was administered with edible oil. Ten mice in each group were killed at 2 and 4 hours after administration and cerebral cortex was collected. Then the level of gamma-aminobutyric acid (GABA), GABA transaminase (GABA-T) activity and GABA receptor (GABA-A and GABA-B) mRNA expression in the cerebral cortex were detected by high performance liquid chromatograph (HPLC), spectrophotometry and real-time RT-PCR respectively. Results GABA levels(μmol/g) in the cerebral cortex of mice administrated with 80 mg/kg of beta-cypermethrin at 2 and 4 hours after the treatment were both higher than those of the control group ((99.77±13.80) vs. (72.10±20.51), (108.29±29.67) vs. (72.09±20.49), both P<0.05). And GABA-T activity (nmol/min.mg.pro) in the cerebral cortex of mice administrated with 40 mg/kg and 80 mg/kg of beta-cypermethrin at 2 hours after the treatment ((12.45±2.20), (11.48±1.33)) were both lower than that of the control group ((14.09±1.64), P<0.05 and P<0.01). Four hours after the administration, the GABA-A mRNA expression in the cerebral cortex of mice administrated with 80 mg/kg of beta-cypermethrin was significantly decreased compared with the control group ((0.89±0.07) vs. (1.00±0.08), P<0.05), while the GABA-B mRNA expression was not statistically different (P＞0.05). Conclusions Beta-cypermethrin can decrease GABA-T activity and increase GABA levels in the cerebral cortex of mice. And then the expression of GABA-A receptor mRNA is inhibited by feedback.
鲍清，杨光，任亚浩，赵越，安丽. 乙体氯氰菊酯对小鼠大脑皮质γ-氨基丁酸水平及相关酶和受体的影响[J]. 实用预防医学, 2017, 24(11): 1300-1302.
BAO Qing, YANG Guang, REN Ya-hao, ZHAO Yue, AN Li. Effects of beta-cypermethrin on γ-aminobutyric acid level, relevant enzyme and receptor in the cerebral cortex of mice. , 2017, 24(11): 1300-1302.
2017, Vol. 24 
