Abstract：Objective To investigate whether resveratrol (RES) could exert anti-osteoarthritis (anti-OA) effect on SW1353 cell line induced by interleukin-1β (IL-1β) via TLR4/MyD88 dependent signaling pathway. Methods The effects of RES(0-100 μmol/L)and IL-1β(10 ng/ml)on the proliferation viability of SW1353 cells were determined by MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt). The SW1353 cells induced by IL-1β (10 ng/ml) were treated by RES (12.5 μmol/L, 50 μmol/L), the level of interleukin-6 (IL-6) in the supernatant of the culture media was measured by ELISA and the expression of Toll-like receptor 4 (TLR4) and myeloid differentiation factor 88 (MyD88) protein was examined by Western blot. Results MTS showed that IL-1β (10 ng/ml) had no significant effect on the cell proliferation viability, while RES at 3.125-25 μmol/L could significantly enhance the cell proliferation viability (P<0.05) and RES at 100 μmol/L could significantly inhibit the cell proliferation viability (P<0.05). IL-1β could up-regulate IL-6 level in the supernatant of culture media in a time-dependent manner (P<0.05), and increase the expression of TLR4 and MyD88 protein in SW1353 cells (P<0.05). RES treatment could dramatically down-regulate IL-6 level in the supernatant of culture media (P<0.05) as well as the expression of TLR4 and MyD88 protein in SW1353 cells (P<0.05). Conclusions Resveratrol may prevent IL-1β-induced osteoarthritis in SW1353 cell line by inhibiting the TLR4/MyD88 dependent signaling pathway. It has potential value in osteoarthritis prevention and treatment.
刘旭丹，于晓璐，赵越，徐小磊，姜梦琪，何建宜，刘莉. 白藜芦醇通过TLR4/MyD88依赖性信号通路对SW1353细胞发挥抗骨关节炎作用的实验研究[J]. 实用预防医学, 2017, 24(11): 1296-1299.
LIU Xu-dan, YU Xiao-lu, ZHAO Yue, XU Xiao-lei, JIANG Meng-qi, HE Jian-yi, LIU Li. Anti-osteoarthritis effect of resveratrol on SW1353 cell line via TLR4/MyD88 signaling pathway: an in vitro study. , 2017, 24(11): 1296-1299.
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