Application of US3 gene in detection of human cytomegalovirus infection collected from late jaundice infants
HU Hong-bo, Chen Kun, PENG Qiao-ying, GUO Hong
1a. Department of Clinical Laboratory; 1b. Department of Neonatology, Hubei Maternal and Child Health Hospital,Wuhan 430070, China; 2. The Ninth Hospital of Wuhan, Wuhan 430080, China
Abstract：To explore the application of US3 gene in detection of human cytomegalovirus infection collected from late jaundice infants. Methods Nested PCR was performed to amplify the US3 gene region of clinical strains collected from late jaundice infants. By comparing with the results of serum HCMV-IgM,HCMV-DNA PCR and HCMV-pp65 antigenemia assay, the coincidence degree of the results in detection of human cytomegalovirus strains collected from late jaundice infants was analyzed. Results ① 145 specimens of late jaundice infants were detected , the positive specimens detected by serum HCMV-IgM were 1, detected by PCR HCMV-DNA and HCMV-pp65 were 24 and 25 respectively. By comparison, the positive specimens detected by the US3 HCMV-DNA were 24. ② There was no significant difference in detection of HCMV infection between US3 gene and PCR HCMV-DNA( P = 1.000). The consistent rate of US3 gene and HCMV-DNA PCR was 97.2% and the value of Kappa was 0.900; There was no significant difference in detection of HCMV infection between US3 gene and HCMV-pp65 ( P = 1.000). The consistent rate of US3 gene and HCMV-pp65 was 95.2% and the value of Kappa was 0.828;③The sensitivity of US3 gene in detection of HCMV strains collected from late jaundice infants was 85.2%, the specificity was 99.2%,Youden index was 84.4, PVP and PVN were 0.958 and 0.967 respectively. ④ The sensitivity of US3 gene in detection of HCMV strains collected from late jaundice infants was 84.0%, the specificity was 97.5%,Youden index was 81.5, PVP and PVN were 0.875 and 0.967 respectively. Conclusions For optimal sensitivity and specificity of the diagnostic method, the parallel use of primers from the US3 gene is recommended.
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