Abstract：Objective： To observe the expression of TGF-β1 ,Bcl-2 in experimental ischemia and perfusion in rat parotid gland conduct epithelial cells,to investigate the relationship between the expression and morphological change.Methods :Ninety rats were performed the bilateral neck arteries tied and divided into 9 groups randomly. The morphological changes of parotid gland and the expression of TGF-β1 Bcl-2 were observed by using light microscope(LM) and immunohistochemistry. Results：Under the condition of the same perfusion time, the expression of TGF-β1 increased at perfusion 0.5 h and 1h Under the condition of the same ischemia time, expressions of TGF-β1 and Bcl-2 didn’t change obviously at early and middle, last phases; but the expression of Bcl-2 increased at early phase only.. At the earlier and middle stage of parotid injured of ischemia and perfusion,the parotid acinar cells showed morphological changes. At last the experimental group underwent repair. Conclusion： Under the condition of ischemia and,expression of TGF-β1 in parotid gland conduct epithelial cells was increased each phase ,but that of Bcl-2 was increased at early phase. Times of Perfusion may not be important factor which effects expression of TGF-β1 in parotid gland conduct epithelial cells The expression of Bcl-2 in parotid gland conduct epithelial cells may not be important factors which be responsible for the histological injures and repaired at ischemia reperfusion .
马宏伟, 王春艳, 吕翔, 吕美娃. 缺血再灌注对大鼠腮腺导管细胞TGF-β1、Bcl-2表达的影响[J]. 实用预防医学, 2016, 23(1): 99-102.
MA Hong-wei, WANG Chun-yan, LV meiwa. Expression of TGF-β1 and Bcl-2 on parotid gland conduct epithelial cells in rat with experimental perfusion after ischemia. , 2016, 23(1): 99-102.
2016, Vol. 23 
