Abstract：Objective s To identify the first gastroenteritis outbreak of GII.8 norovirus during May to June in Fengtai of Beijing and further analyze the genotypes of the pathogens. Methods 20 samples were collected to detected NVs by real-time fluorescent quantitative PCR. The positive samples were amplified by one-step RT-PCR, then the amplification products were sequenced for nucleotide sequence alignments and phylogenetic analysis with BioEdit and MEGA 5.2.2 software. Results 12 samples were identified GII NVs positive with a 60.00% positive rate.The alignment and phylogenetic analysis revealed that the outbreak of gastroenteritis were caused by GII.8 NVs. Conclusions The outbreak of norovirus induced infectious diarrhea was probably due to the consumption of close contact between students．It is the first time found that the outbreak of gastroenteritis were caused by GII.8 NVs in Fengtai, and need to strengthen the monitoring of NVs .
余红, 秦萌, 尉秀霞, 陆翌禹, 杨军勇, 武庆锐, 董晓根, 李洁. 北京市丰台区首起GII.8诺如病毒暴发疫情的实验室鉴定及基因特征分析[J]. 实用预防医学, 2016, 23(5): 618-620.
YU Hong, QIN Meng, Wei Xiu-xia, LU Yi-yu, YANG Jun-yong, WU Qing-rui, DONG Xiao-gen, LI Jie.. Etiological analysis and genotyping detected in the first outbreak of GII.8 norovirus of gastroenteritis in Beijing Fengtai.. , 2016, 23(5): 618-620.
2016, Vol. 23 
