Abstract：Objective To explore the significance of combined detection of serum Golgi protein 73 (GP73), heat shock protein 27 (HSP27) and α-fetoprotein (AFP) in the diagnosis of hepatitis B virus-related early hepatocellular carcinoma. Methods Enzyme-linked immunosorbent assay (ELISA) was used to detect serum GP73 and HSP27 and electrochemical luminescence immunoassay to determine AFP in 50 patients with HBV-related early hepatocellular carcinoma (HCC), 50 patients with HBV-related cirrhosis and 30 healthy controls. Real-time fluorescent quantitative PCR assay was employed to detect the level of HBV-DNA in patients with early HCC. Results The serum levels of GP73, HSP27 and AFP in the early HCC patients were (266.37±98.41)ng/ml, (847.18± 211.72) pg/ml and (368.56±65.32) ng/ml respectively, which were all significantly higher than those in the liver cirrhosis group [(60.23±32.30)ng/ml, (338.46±114.3)pg/ml and (26.22±8.58) ng/ml respectively] and the healthy control group [(23.32±11.08)ng/ml,(73.39±38.54)pg/ml and(7.15±3.32)ng/ml respectively], with statistically significant differences (all P<0.01). The positive rates of single test of serum AFP, GP73 and HSP27 and the positive rate of combined detection of AFP+GP73+HSP27 in the early HCC patients were 75%, 82% 74% and 86% respectively, which were all significantly higher than those of the liver cirrhosis group (48%, 37.5%, 18% and 32% respectively) and the healthy control group (3%, 6%, 0% and 0% respectively) (all P<0.01), The positive rate of combined detection of AFP+GP73+HSP27 was the highest (P<0.01). The correlation coefficients between AFP, GP73, HSP27 and HBV-DNA was 0.076, 0.119 and 0.176 respectively (P>0.05). Conclusions Combined detection of GP73, HSP27 and AFP can be used as an important indicator for diagnosis of HCC and provides a basis for its early detection and treatment. But no correlation is found between the level of HBV-DNA and GP73, HSP27 and AFP.
黎晓武, 李金强,罗向波, 侯琴, 何珊. 血清GP73、HSP27联合AFP检测在HBV相关早期肝细胞癌诊断的价值[J]. 实用预防医学, 2016, 23(11): 1319-1321.
LI Xiao-wu, LI Jin-qiang, LUO Xiang-bo, HOU Qin, HE Shan. Value of combined detection of serum GP73, HSP27 and AFP in the diagnosis of hepatitis B virus-associated early hepatocellular carcinoma. , 2016, 23(11): 1319-1321.
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2016, Vol. 23 
