Identification and analysis of Bacillus cereus in distillers grains facial masks
XIAO Ye1, CHEN Cheng2, GAO Qiong3
1. Yiyang Institute for Food and Drug Control, Yiyang, Hunan 413046, China; 2. Hunan Products Evaluation Center, Changsha, Hunan 410029, China; 3. Hunan Provincial Center for Disease Control and Prevention, Changsha, Hunan 410005, China
Abstract：Objective To identify the suspected Bacillus cereus detected in facial mask cosmetics, to analyze the reasons for the unqualified total colony count of facial masks, and to provide references and suggestions for preventing Bacillus cereus contamination in facial mask products and unqualified microbial items. Methods The morphology observation, biochemical identification and VITEK-2 confirmation of the suspected bacteria found in the inspection of facial masks were carried out with reference to the national standards like the Technical Specifications for the Safety of Cosmetics (2015 version), GB 4789.14-2014 National Food Safety Standard Microbiological Examination of Bacillus cereus and the commodity inspection standard SN/T 2206.2-2009 Microbiological Examination Methods of Cosmetics Part 2: Aerobic Spore-Bearing Bacillus and Bacillus cereus. Results The morphological characteristics of the bacteria on nutrient agar plates and MYP plates were consistent with those of Bacillus cereus. Based on the test results of biochemical identification kit, VITEK-2 confirmation results, and root test, hemolysis test, and protein toxin crystallization test results of the bacteria, it could be concluded that the bacteria were Bacillus cereus. Conclusion Distillers grains facial masks were contaminated by Bacillus cereus, which might be one of the reasons for the unqualified item of the total bacterial count. The results suggest that cosmetics manufacturers should pay attention to preventing Bacillus cereus contamination when adding food crops to their products.
2023, Vol. 30 
