Genetic characteristics of hemagglutinin and neuraminidase of avian influenzaA (H7N9)virus from one human infection case in a district of Beijing
WU Yang1, NIU Huan-cai1, ZHUANG Peng1, LI Dong-xun1, LIU Zhong-cheng1, LI Sa1, SHU Gao-lin1, PAN Yang2
1. Changping District Center for Disease Control and Prevention, Beijing 102200, China; 2. Beijing Center for Disease Control and Prevention, Beijing 100013, China
Abstract：Objective To study the genetic characteristics of avian influenza A (H7N9)virus from one human infection case in a district of Beijing so as to provide references for early detection of avian influenza cases and control of the epidemic. Methods Nucleic acid of clinical sample of the case was extracted and detected by one-step RT-PCR. After the reverse transcription amplification, the coding regions of hemagglutinin (HA) and neuraminidase (NA) genes were sequenced. The results of sequencing were compared with their corresponding sequence in Global Initiative on Sharing All Influenza Data (GISAID) database, and homology analysis of the virus strain in this district and other ones previously detected in Beijing and other provinces and cities was conducted. A phylogeny tree was constructed. Sequence alignment and homology-modeling methods were used to study specific mutations regarding phenotypes, specifically addressing the human receptor binding properties. Results The results demonstrated that it was a low-pathogenic strain. There were minor differences in HA and NA proteins between 29 human H7N9 strains previously detected in Beijing and the strain found in this district, with the homology of 97.2%-100% and 96.8%-100%, respectively. The strain was highly homologous to candidate vaccine viruses (A/Hunan/02650/2016) based on phylogenetic analysis. Viral amino acid variation showed that this strain had mutations at 186 (G186V) and 226 (Q226L) sites in HA protein. The HA and NA glycosylation sites were conservative. Conclusions The sequences of H7N9 virus strain found in this district are highly homologous to those of the virus strains previously detected in Beijing. Though the strains have some significant mutations of amino acid in HA and NA proteins, all isolates show no drug-resistant mutations.
吴杨, 牛桓彩, 庄鹏, 李东迅, 刘重程, 李飒, 舒高林, 潘阳. 北京市某区1例人感染H7N9禽流感病毒的HA和NA基因特征分析[J]. 实用预防医学, 2020, 27(6): 667-671.
WU Yang, NIU Huan-cai, ZHUANG Peng, LI Dong-xun, LIU Zhong-cheng, LI Sa, SHU Gao-lin, PAN Yang. Genetic characteristics of hemagglutinin and neuraminidase of avian influenzaA (H7N9)virus from one human infection case in a district of Beijing. , 2020, 27(6): 667-671.
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2020, Vol. 27 
