Abstract：Objective To investigate the inapparent infection of hantavirus (HV) in healthy population in Hebei Province, and to valuate the immune efficacy of a bivalent vaccine against hemorrhagic fever with renal syndrome (HFRS). Methods Luannan County, Beidaihe County and Changli County were selected as the vaccination points, and the healthy people aged 16-60 years except those suffered from HFRS previously and those with contraindications served as the inoculable objects. The titers of neutralizing antibody (NA) were detected by the fluorescent antibody virus neutralization (FAVN) assay before and after the immune inoculation. Results The positive rate of neutralizing antibody in the population after vaccination was significantly higher than that before vaccination (70.42% vs. 2.92%, P<0.05). If was not significantly different among different age groups before vaccination (P>0.05), but it was after vaccination (P<0.05). Moreover, the positive rates of neutralizing antibody after vaccination in each age group and the total population were all significantly higher than those before (P<0.001). The titer of neutralizing antibody after vaccination was increased, but no statistically significant differences were found among different age groups (P>0.05). Conclusions The double-titer inactivated vaccine against HFRS has obvious immune efficacy. It is worth while spreading widely.
2017, Vol. 24 
