Abstract：Objective To explore the expression of Galectin-3 gene in hepatocellular carcinoma cells and the effect and mechanism of inhibiting its expression on the proliferation and invasion of hepatocellular carcinoma cells. Methods Reverse transcription-polymerase chain reaction (RT-PCR) assay was used to detect the mRNA expression of Galectin-3 gene in human hepatocellular carcinoma cell lines MHCC-97H, HepG2 and SMCC-7721 and human normal liver cell line HL-7702. Control, NC-siRNA and Galectin-3-siRNA were transfected into HepG2 cells, and Western blot was employed to detect Galectin-3, MMP-2, MMP-9, Notch1 and Hes1 protein expression after 48 hours. Cell counting kit-8(CCK8) assay and flow cytometry were used to respectively detect the cell proliferation and invasiveness. Results The mRNA expression of Galectin-3 in MHCC-97H, HepG2 and SMCC-7721 cells was significantly higher than that in normal liver cell line HL-7702, showing statistically significant differences (all P<0.01). The expression of Galectin-3 in HepG2 cells was the highest, which was selected as the follow-up research object. After siRNA being transfected, the expression of Galectin-3 was significantly inhibited. The cell survival rate,invasive cell-counting and MMP-2, MMP-9, Notch1 and Hes1 protein expression in Galectin-3-siRNA group were all lower than those of the control group and NC-siRNA group, with statistically significant differences (all P<0.01). Conclusions Inhibition of Galectin-3 gene expression in hepatocellular carcinoma cells can significantly decline the proliferation and invasion of cancer cells, and its mechanism is related to the regulation of Notch1 signaling pathway.
徐玉玲,罗宏伟,丁沛,孟祥林,李海雷. 肝癌细胞中抑制Galectin-3基因表达对细胞增殖及侵袭能力的影响及机制研究[J]. 实用预防医学, 2018, 25(4): 421-424.
XU Yu-ling, LUO Hong-wei, DING Pei, MENG Xiang-lin, LI Hai-lei. Effect and mechanism of inhibition of Galectin-3 gene expression on proliferation and invasion of hepatocellular carcinoma cells. , 2018, 25(4): 421-424.
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