Comparison of Antibody Titers Measured by Two Evaluation Methods
LI Jiang-jiao, DU Hui-jing, CHEN Chi, SHI Ji-chun, XU Miao, YE Qiang
Key Laboratory for Standardization of Methods for Quality Control of Biotechnical Products, Ministry of Health, National Institutes for Food and Drug Control, Beijing 100050, China Corresponding author: YE Qiang, E-mail：qiangyee@nicpbp.org.cn
Abstract：To compare and analyze the correlation of opsonophagocytic antibody titers and the antibody concentrations of sera samples obtained after 13-valent pneumococcal conjugate vaccine immunization. Methods The opsonophagocytic titers of sera against 13 pneumococcal serotypes were determined using opsonophagocytic assay (OPA) and compared with the corresponding ELISA titers,between which the relationship was analyzed for each pneumococcal serotype．Results The correlation coefficients were high for serotypes 1,3,5,6A,6B,9V,14,18C,19A and 23F, ranged from 0.71 to 0.88. The correlation coefficients were relatively low for serotype19F and 4, were 0.66 and 0.52 respectively. Conclusion The relationship between the opsonophagocytic activity and antibody concentration for 13-valent Pneumococcal conjugate vaccine shows a good correlation.
2014, Vol. 21 
