Abstract：Objective:In this paper, PCR analysis were used to detect the virulence genes of Listeria monocytogenes in delicatessen in order to provide scientific evidence for risk assessment in Shunyi District of Beijing from 2013 to 2014. Methods:Delicatessen was collected randomly from markets, the isolation of Listeria monocytogenes was performed according to the GB/T 4789.30-2010. At the same time, the genes of 16sRNA, hlyA, ipa60, dth-18 were detected in the isolates. Results:A total of 6 Listeria monocytogenes were isolated from 180 samples. The genes of 16sRNA and dth-18 were found in all the isolates and hlyA and ipa60 were found in 5 of them. The sensitivity of these primers was different slightly. Conclusions:The results indicated that the delicatessen in markets of Shunyi District was contaminated by Listeria monocytogenes to a certain extent. 83.33% (5/6) isolates were hlyA+/ipa60+ strains.
李长青,邵占涛,李颖,王彦波,王园园,朱美娟. 2013-2014年北京市顺义区熟肉制品中单核细胞增生李斯特氏菌致病因子检测分析[J]. 实用预防医学, 2015, 22(8): 936-937.
LI Chang-qing, SHAO Zhang-tao, LI Ying, WANG Yan-bo, WANG Yuan-yuan, ZHU Mei-juan. PCR analysis for virulence genes of Listeria monocytogenes in delicatessen in Shunyi District, Beijing, from 2013 to 2014.. , 2015, 22(8): 936-937.
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2015, Vol. 22 
