Abstract：Objective: Compare new domestic parasites nucleic acid real-time fluorescent PCR rapid detection reagent (hereinafter referred to as the RT-PCR detection reagent) and the thickness of the gold standard blood membrane microscope (hereinafter referred to as the "microscope method") and research both of them. Methods: Extract the DNA of whole blood samples, add RT-PCR detection reagent for fluorescence quantitative PCR detection and compare related indicators. Results：Two methods by paired comparison, the total coincidence rate of 45 whole blood clinical samples is 93.33% by RT-PCR. 3 cases of microscope method test is negative, after testing positive by RT-PCR detection reagent contain Plasmodium DNA sequences by sequencing detection of samples. Conclusions: New method of RT-PCR detection reagent and microscopy there is no significant difference in the indicators. The real-time fluorescent PCR reagents have good detection quality, especially suitable for the port, entry-exit inspection and quarantine department Plasmodium DNA detection.
鲁少平，邓中平，龙妍娇，陆雯，付亚成，王晓丽. 运用实时荧光RT-PCR方法检测疟原虫的研究[J]. 实用预防医学, 2015, 22(10): 1171-1173.
Lu Shao-Ping, Deng Zhong-Ping, Long Yan-Jiao. Real-Time Reverse Transcription PCR on Detection of Plasmodium. , 2015, 22(10): 1171-1173.
2015, Vol. 22 
