Abstract：Objective To explore the relationship of two different nucleic acid extraction methods with the minimum detection limit of Epstein-Barr virus (EBV) DNA in peripheral blood of patients with nasopharyngeal carcinoma (NPC). Methods The nucleic acid release agent method and spin columns method were used to measure EBV-DNA load in 40 peripheral blood specimens from NPC patients, their detection rates were compared, and their minimum detection limits were identified. Results Among the 40 whole blood samples detected, the EBV-DNA-positive detection rate of the nucleic acid release agent method was significantly higher than that of the spin columns method, with a statistically significant difference (90.0% vs. 62.6%, χ2=8.352, P=0.008). The national professional standard verified that the minimum detection limits of EBV-DNA load by the nucleic acid release agent method and spin columns method were 400 copies/ml and 900 copies/ml respectively. Conclusions Quantification of EBV in peripheral blood of NPC patients should use the nucleic acid extraction kit by which the requirement of the minimum detection limit can be reached or surpassed. The appropriate reagent canObjectively reflect the change of EBV in the blood, and provide exact and reliable data for clinical diagnosis and treatment of EBV-associated NPC.
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2016, Vol. 23 
