Simultaneous determination of seven functional components in health-care food from Chinese herbal medicine by ultrahigh performance liquid chromatography
YANG Zhen, HU Cong, WANG Qiong
Changsha Municipal Center for Disease Control and Prevention, Changsha, Hunan 410000, China
Abstract：Objective To establish a sensitive and convenient analytical method based on ultrahigh performance liquid chromatography (UPLC) for simultaneous determination of seven functional components (including tanshinone IIA, paeoniflorin, jasminoside, paeonol, rutin, icariin and magnoflorine) in health-care food from Chinese herbal medicine. Methods The analytes were extracted by ultrasound with 60% methanol and separated by a C18 column. The mobile phase was methanol-0.1% formic acid aqueous solution with gradient elution. Uv-wavelength detection was performed, and external standard method was used for the quantitative analysis. Results Tanshinone IIA, paeoniflorin, jasminoside, rutin and magnoflorine showed good linearity when the concentration ranged from 0.50 mg/L-50.0 mg/L, while paeonol and icariin displayed good linearity over the concentration range of 0.10 mg/L-25.0 mg/L, with the correlation coefficients r≥0.9992. The method detection limits for the seven compounds were 0.1 mg/kg-1 mg/kg. The recoveries ranged from 88.9% to 105.0%. The relative standard deviations (RSDs) were in the range of 0.4%-7.7%. Conclusion The proposed method is simple, sensitive and rapid, and it is suitable for simultaneous determination of the seven functional components in health-care food from Chinese herbal medicine.
杨珍, 胡骢, 汪琼. 超高效液相色谱法同时测定中草药保健食品中7种功效成分[J]. 实用预防医学, 2023, 30(1): 66-68.
YANG Zhen, HU Cong, WANG Qiong. Simultaneous determination of seven functional components in health-care food from Chinese herbal medicine by ultrahigh performance liquid chromatography. , 2023, 30(1): 66-68.
2023, Vol. 30 
