Abstract：Objective To establish a method for simultaneous determination of 8 water-soluble dyes including quinoline yellow, acid orange 12, basic orange 21, basic orange 22, acid red 2G, azorubin, bright black, and fluorescent pink in meat and meat products by solid phase extraction combined with ultrahigh performance liquid chromatography. Methods The samples were extracted with a simple ultrasonic extraction procedure with 20% methanol and cleaned up on a C18 cartridge. The analyte was separated by C18 column with the mobile phase consisting of methanol-ammonium acetate solution under gradient elution mode and detected by diode array detector. Results Under the optimized detection conditions, a good linear relationship was achieved as the massconcentrations of the 8 water-soluble dyes ranged from 0.050 mg/L to 50.0 mg/L, and the correlation coefficient (r) was ≥ 0.9990. The method detection limits were between 0.008 mg/kg and 0.06 mg/kg. Satisfactory recoveries were obtained with the values of 81.1%-108.4%, and the relative standard deviations (RSDs) were 1.8%-6.1%. The method developed was applied to the determination of the 8 water-soluble dyes in 23 samples of meat and meat products. Acid red 2G was detected in one sample of sausage, and alkaline orange 21 was found in one sample of fish, with the values being 2.48 mg/kg and 2.09 mg/kg, respectively. Conclusion The method developed is sensitive and accurate, and it is suitable for simultaneous determination of the 8 water-soluble dyes in meat and meat products.
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2023, Vol. 30 
