Abstract：Objective To develop a multiplex polymerase chain reaction (PCR) assay for rapid identification of Escherichia albertii. Methods The multiplex PCR system was established based on lysP and EA0134 genes. Through optimizing the primer concentration, reaction time and temperature and other reaction conditions, the multiplex PCR system was evaluated by using 63 strains of Escherichia albertii, Escherichia albertii and non-Escherichia albertii enrichment broth.Results The 2 pairs of primers could specifically amplify the corresponding genes, and the sensitivity was both 25 CFU/reaction (500 CFU/ml) and very specific. The detection Results of enrichment broth of Escherichia albertii and non-Escherichia albertii isolated in this experiment were consistent with those previous conclusions drawn by the conventional assay. Conclusions The multiplex PCR system established in this study can be used to preliminary screen Escherichia albertii and identify the isolates, and this study provides an effective technological method for rapid identification of Escherichia albertii.
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2018, Vol. 25 
