Abstract：Objective To investigate the genotypes and molecular epidemiological characteristics of norovirus in Zhuhai region during 2011-2013. Methods We collected 619 anal swab specimens from patients during acute gastroenteritis outbreaks and 1,150 stool specimens from children hospitalized for diarrhea in 2 surveillance hospitals in Zhuhai region from 2011 to 2013. Norovirus antigen was detected by ELISA. Some norovirus antigen-positive stool specimens were selected to amplify the N/S gene by RT-PCR, and then PCR products were sequenced and genotyped. Results Among 28 outbreaks of acute gastroenteritis, 17 ere positive for norovirus, and 19.55%(121/619) of the anal swab specimens were positive for norovirus antigen. 47 specimens which were successfully genotyped belonged to norovirus genogroup II (Nov G II). 14 strains belonged to Nov G II.4, including 5 Nov G II.4 2006b strains and 9 Nov G II.4/Sydney_2012 strains. 12 strains belonged to Nov G II.3, 3 strains Nov G II.5 and 18 strains Nov G II.6. 33.13%(381/1,150) of the stool specimens were positive for norovirus antigen. 71 specimens which were successfully genotyped belonged to Nov G II. 36 strains belonged to Nov G II.4 2006b, 32 strains Nov G II. 4/Sydney_2012, 2 strains Nov G II.3 and 1 strain Nov G II.1. Conclusions Nov GII is prevailing in the outbreaks of acute gastroenteritis in Zhuhai City, but the genotypes are widely distributed. Nov G II.4 is identified as the predominant genotype among children with norovirus infection in Zhuhai City.
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2017, Vol. 24 
